Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BMB171_RS21990 Genome accession   NC_014171
Coordinates   4276810..4279149 (-) Length   779 a.a.
NCBI ID   WP_000941229.1    Uniprot ID   A0A9Q5MX00
Organism   Bacillus thuringiensis BMB171     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4271810..4284149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMB171_RS31000 (BMB171_C4068) - 4273057..4273233 (-) 177 WP_001242605.1 hypothetical protein -
  BMB171_RS21975 (BMB171_C4069) - 4273399..4273839 (-) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BMB171_RS21980 (BMB171_C4070) relA 4273856..4276039 (-) 2184 WP_001262795.1 GTP diphosphokinase -
  BMB171_RS21985 (BMB171_C4071) - 4276250..4276762 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  BMB171_RS21990 (BMB171_C4072) recJ 4276810..4279149 (-) 2340 WP_000941229.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BMB171_RS21995 (BMB171_C4073) - 4279251..4280144 (-) 894 WP_000409743.1 cation diffusion facilitator family transporter -
  BMB171_RS22000 (BMB171_C4074) secDF 4280301..4282565 (-) 2265 WP_001119066.1 protein translocase subunit SecDF -
  BMB171_RS22005 (BMB171_C4075) - 4282835..4283119 (-) 285 WP_000886980.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88118.63 Da        Isoelectric Point: 4.9019

>NTDB_id=37315 BMB171_RS21990 WP_000941229.1 4276810..4279149(-) (recJ) [Bacillus thuringiensis BMB171]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=37315 BMB171_RS21990 WP_000941229.1 4276810..4279149(-) (recJ) [Bacillus thuringiensis BMB171]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTCGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACAGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCATTTCGTTGGGCGC
ATAGCGCAGGGTTCTCGCTAATTATTACTGTCGATACTGGTATTGCAGCGGTACATGAAGCGAATGTAGCGAAAGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGATGGCGGTGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATCGCAGTAATCGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAGAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACCGAAGAGAGTATTGGTTTCTCAATTGCACCTCGTATTAATGCGGTTGGGCGTTTAGAAGATGCTGCGC
CGGCTGTACACCTTTTATTATCAGATGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTGGAAAATAACTTCCCACCAGAAGAAAATAAAGT
ATTAGTACTTGCGAAAGAAGGCTGGAACCCAGGGGTAATTGGAATTGTTGCTTCGAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTACCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGCTTAAATGAACAAGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGTAAAGTAGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAAAAACTAGCCCCGTTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTAAAAGATGCAGAGTTAGAAAGCATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGCTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCGTCTATTAATGAATGGAATAACTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGTGAG
CTATTTAAGGTAGGATTCCCAGCGCGAATTTATACGCTATTTTATCAAGAGAATAACCACTTATTTAGTACAGTTCCGAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.326

99.358

0.52

Multiple sequence alignment