Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   G5T40_RS23795 Genome accession   NZ_CP049196
Coordinates   5002848..5003612 (-) Length   254 a.a.
NCBI ID   WP_001136198.1    Uniprot ID   -
Organism   Escherichia coli strain E118     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4997848..5008612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5T40_RS23785 nikR 5001639..5002040 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  G5T40_RS23790 nikE 5002045..5002851 (-) 807 WP_001521512.1 nickel import ATP-binding protein NikE -
  G5T40_RS23795 amiE 5002848..5003612 (-) 765 WP_001136198.1 nickel import ATP-binding protein NikD Regulator
  G5T40_RS23800 nikC 5003612..5004445 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  G5T40_RS23805 nikB 5004442..5005386 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  G5T40_RS23810 nikA 5005386..5006960 (-) 1575 WP_000493127.1 nickel ABC transporter substrate-binding protein -
  G5T40_RS23815 acpT 5007071..5007658 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26843.46 Da        Isoelectric Point: 6.6886

>NTDB_id=372966 G5T40_RS23795 WP_001136198.1 5002848..5003612(-) (amiE) [Escherichia coli strain E118]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=372966 G5T40_RS23795 WP_001136198.1 5002848..5003612(-) (amiE) [Escherichia coli strain E118]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402