Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   G5V60_RS01340 Genome accession   NZ_CP049101
Coordinates   291795..292559 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain EC28     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 286795..297559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS01310 (G5V60_01310) - 287429..288787 (-) 1359 WP_001525857.1 PTS galactitol transporter subunit IIC -
  G5V60_RS01315 (G5V60_01315) - 288864..289145 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  G5V60_RS01320 (G5V60_01320) - 289142..289615 (-) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  G5V60_RS01325 (G5V60_01325) - 289640..290386 (-) 747 WP_001296489.1 UTRA domain-containing protein -
  G5V60_RS01330 (G5V60_01330) nikR 290585..290986 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  G5V60_RS01335 (G5V60_01335) nikE 290992..291798 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  G5V60_RS01340 (G5V60_01340) amiE 291795..292559 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  G5V60_RS01345 (G5V60_01345) nikC 292559..293392 (-) 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
  G5V60_RS01350 (G5V60_01350) nikB 293389..294333 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  G5V60_RS01355 (G5V60_01355) nikA 294333..295907 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  G5V60_RS01360 (G5V60_01360) acpT 296018..296605 (-) 588 WP_000285760.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=372374 G5V60_RS01340 WP_001136232.1 291795..292559(-) (amiE) [Escherichia coli strain EC28]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=372374 G5V60_RS01340 WP_001136232.1 291795..292559(-) (amiE) [Escherichia coli strain EC28]
ATGCCGCAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGAATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGTATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTGACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398