Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   G5C00_RS12310 Genome accession   NZ_CP048934
Coordinates   2507024..2507788 (+) Length   254 a.a.
NCBI ID   WP_001136198.1    Uniprot ID   -
Organism   Escherichia coli strain 190693     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2502024..2512788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5C00_RS12290 (G5C00_12270) acpT 2502978..2503565 (+) 588 WP_000285781.1 4'-phosphopantetheinyl transferase AcpT -
  G5C00_RS12295 (G5C00_12275) nikA 2503676..2505250 (+) 1575 WP_000953368.1 nickel ABC transporter substrate-binding protein -
  G5C00_RS12300 (G5C00_12280) nikB 2505250..2506194 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  G5C00_RS12305 (G5C00_12285) nikC 2506191..2507024 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  G5C00_RS12310 (G5C00_12290) amiE 2507024..2507788 (+) 765 WP_001136198.1 nickel import ATP-binding protein NikD Regulator
  G5C00_RS12315 (G5C00_12295) nikE 2507785..2508591 (+) 807 WP_001521512.1 nickel import ATP-binding protein NikE -
  G5C00_RS12320 (G5C00_12300) nikR 2508596..2508997 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26843.46 Da        Isoelectric Point: 6.6886

>NTDB_id=371818 G5C00_RS12310 WP_001136198.1 2507024..2507788(+) (amiE) [Escherichia coli strain 190693]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=371818 G5C00_RS12310 WP_001136198.1 2507024..2507788(+) (amiE) [Escherichia coli strain 190693]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402