Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FKM99_RS24650 Genome accession   NZ_CP041202
Coordinates   3165827..3166843 (-) Length   338 a.a.
NCBI ID   WP_017449463.1    Uniprot ID   A0A7Y0SFN2
Organism   Vibrio parahaemolyticus strain Vb0624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3160827..3171843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKM99_RS24625 (FKM99_24625) aroK 3161687..3162205 (-) 519 WP_005381319.1 shikimate kinase AroK -
  FKM99_RS24630 (FKM99_24630) pilQ 3162394..3164142 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  FKM99_RS24635 (FKM99_24635) pilP 3164174..3164689 (-) 516 WP_025525190.1 pilus assembly protein PilP Machinery gene
  FKM99_RS24640 (FKM99_24640) pilO 3164679..3165272 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FKM99_RS24645 (FKM99_24645) pilN 3165265..3165843 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  FKM99_RS24650 (FKM99_24650) pilM 3165827..3166843 (-) 1017 WP_017449463.1 type IV pilus biogenesis protein PilM Machinery gene
  FKM99_RS24655 (FKM99_24655) - 3166986..3169556 (+) 2571 WP_140268153.1 penicillin-binding protein 1A -
  FKM99_RS24660 (FKM99_24660) oxyR 3169612..3170520 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FKM99_RS24665 (FKM99_24665) - 3170691..3171419 (+) 729 WP_141180474.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.26 Da        Isoelectric Point: 6.8322

>NTDB_id=370476 FKM99_RS24650 WP_017449463.1 3165827..3166843(-) (pilM) [Vibrio parahaemolyticus strain Vb0624]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=370476 FKM99_RS24650 WP_017449463.1 3165827..3166843(-) (pilM) [Vibrio parahaemolyticus strain Vb0624]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0SFN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment