Detailed information NTG0369760

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FIM06_RS12420
• Genome accession: NZ_CP041144
• Coordinates: 2534759..2535373 (-)
• Length: 204 a.a.
• NCBI ID: WP_105937505.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain UCMB5044
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2529759..2540373

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIM06_RS12390	rpsT	2530062..2530328 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
FIM06_RS12395 (FIM06_2312)	holA	2530361..2531386 (-)	1026	Protein_2377	DNA polymerase III subunit delta	-
FIM06_RS12400	-	2531426..2531577 (-)	152	Protein_2378	hypothetical protein	-
FIM06_RS12405	-	2531618..2531752 (+)	135	WP_003152870.1	YqzM family protein	-
FIM06_RS12410 (FIM06_2313)	comEC	2531771..2534122 (-)	2352	WP_105937506.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FIM06_RS12415 (FIM06_2314)	-	2534123..2534692 (-)	570	WP_003152868.1	ComE operon protein 2	-
FIM06_RS12420 (FIM06_2315)	comEA	2534759..2535373 (-)	615	WP_105937505.1	helix-hairpin-helix domain-containing protein	Machinery gene
FIM06_RS12425 (FIM06_2316)	comER	2535432..2536253 (+)	822	WP_012118027.1	late competence protein ComER	-
FIM06_RS12430 (FIM06_2317)	-	2536322..2537059 (-)	738	WP_032873951.1	class I SAM-dependent methyltransferase	-
FIM06_RS12435 (FIM06_2318)	rsfS	2537056..2537412 (-)	357	WP_007408260.1	ribosome silencing factor	-
FIM06_RS12440 (FIM06_2319)	yqeK	2537430..2537990 (-)	561	WP_140957459.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
FIM06_RS12445 (FIM06_2320)	-	2537980..2538549 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
FIM06_RS12450 (FIM06_2321)	yhbY	2538560..2538850 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
FIM06_RS12455 (FIM06_2322)	aroE	2538844..2539686 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22206.45 Da
• Isoelectric Point: 6.4940

>NTDB_id=369760 FIM06_RS12420 WP_105937505.1 2534759..2535373(-) (comEA) [Bacillus velezensis strain UCMB5044]
MTKAMMTERLRRNLKILGACLVAGAVFICILLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTVEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=369760 FIM06_RS12420 WP_105937505.1 2534759..2535373(-) (comEA) [Bacillus velezensis strain UCMB5044]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTTGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGTTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATAACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.592	93.627	0.539
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363
comEA	Latilactobacillus sakei subsp. sakei 23K	32.314	100	0.363