Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   FJM50_RS27070 Genome accession   NZ_CP041066
Coordinates   5010199..5012541 (-) Length   780 a.a.
NCBI ID   WP_140457054.1    Uniprot ID   -
Organism   Priestia megaterium strain KNU-01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5005199..5017541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJM50_RS27045 - 5005592..5005768 (-) 177 WP_013059305.1 hypothetical protein -
  FJM50_RS27050 - 5005935..5006819 (+) 885 WP_223268964.1 N-acetylmuramoyl-L-alanine amidase -
  FJM50_RS27055 dtd 5006810..5007271 (-) 462 WP_013059307.1 D-aminoacyl-tRNA deacylase -
  FJM50_RS27060 - 5007287..5009485 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  FJM50_RS27065 - 5009671..5010183 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  FJM50_RS27070 recJ 5010199..5012541 (-) 2343 WP_140457054.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  FJM50_RS27075 secDF 5012683..5014920 (-) 2238 WP_140457055.1 protein translocase subunit SecDF -
  FJM50_RS27080 - 5015058..5015354 (-) 297 WP_098279047.1 post-transcriptional regulator -
  FJM50_RS27085 spoVB 5015478..5017028 (+) 1551 WP_025601348.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87907.51 Da        Isoelectric Point: 4.7240

>NTDB_id=369326 FJM50_RS27070 WP_140457054.1 5010199..5012541(-) (recJ) [Priestia megaterium strain KNU-01]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESMYPPDENSVLIVEGEEWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQDEEQAKACMFAGEQIVLLDLPPSQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=369326 FJM50_RS27070 WP_140457054.1 5010199..5012541(-) (recJ) [Priestia megaterium strain KNU-01]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGCATTCAGCAGGCAATTGATCATAAT
GAAAAAATTATGATTTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AATTTTGATTTAATTATTACCGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGATTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATTGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCCGATC
CAGCTGTTCATTTGCTTATGACGACAAATGCTGAAGAGGCAAAAGGCTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAGGAAGCCGTTGAATTTGTTGAGAGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGAATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAATTCTATAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGTTTTGACTTATTC
GCGAACTTATCTAAGTGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTAAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAGATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAATGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTAACAGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAGAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.641

100

0.556

Multiple sequence alignment