Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GZS15_RS01350 Genome accession   NZ_CP048322
Coordinates   302325..303089 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain 64     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 297325..308089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS15_RS01325 (GZS15_01325) yhhJ 299181..300305 (+) 1125 WP_001314210.1 ABC transporter permease -
  GZS15_RS01330 (GZS15_01330) - 300409..300639 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  GZS15_RS01335 (GZS15_01335) - 300636..300995 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  GZS15_RS01340 (GZS15_01340) nikR 301115..301516 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  GZS15_RS01345 (GZS15_01345) nikE 301522..302328 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  GZS15_RS01350 (GZS15_01350) amiE 302325..303089 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  GZS15_RS01355 (GZS15_01355) nikC 303089..303922 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GZS15_RS01360 (GZS15_01360) nikB 303919..304863 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GZS15_RS01365 (GZS15_01365) nikA 304863..306437 (-) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  GZS15_RS01370 (GZS15_01370) acpT 306548..307135 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=367755 GZS15_RS01350 WP_001136236.1 302325..303089(-) (amiE) [Escherichia coli strain 64]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=367755 GZS15_RS01350 WP_001136236.1 302325..303089(-) (amiE) [Escherichia coli strain 64]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398