Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   FHE73_RS22955 Genome accession   NZ_CP040782
Coordinates   4425053..4427392 (-) Length   779 a.a.
NCBI ID   WP_089148266.1    Uniprot ID   A0A9X6RV09
Organism   Bacillus thuringiensis strain HM-311     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4420053..4432392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHE73_RS31320 - 4420935..4421111 (-) 177 WP_001242605.1 hypothetical protein -
  FHE73_RS22935 (FHE73_22935) - 4421194..4421526 (-) 333 WP_000938749.1 YrdB family protein -
  FHE73_RS22940 (FHE73_22940) - 4421642..4422082 (-) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  FHE73_RS22945 (FHE73_22945) relA 4422099..4424282 (-) 2184 WP_001262803.1 GTP diphosphokinase -
  FHE73_RS22950 (FHE73_22950) - 4424493..4425005 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  FHE73_RS22955 (FHE73_22955) recJ 4425053..4427392 (-) 2340 WP_089148266.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  FHE73_RS22960 (FHE73_22960) - 4427494..4428387 (-) 894 WP_000409743.1 cation diffusion facilitator family transporter -
  FHE73_RS22965 (FHE73_22965) secDF 4428544..4430808 (-) 2265 WP_001119051.1 protein translocase subunit SecDF -
  FHE73_RS22970 (FHE73_22970) - 4431078..4431362 (-) 285 WP_000886980.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88148.70 Da        Isoelectric Point: 4.9034

>NTDB_id=367147 FHE73_RS22955 WP_089148266.1 4425053..4427392(-) (recJ) [Bacillus thuringiensis strain HM-311]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNLGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=367147 FHE73_RS22955 WP_089148266.1 4425053..4427392(-) (recJ) [Bacillus thuringiensis strain HM-311]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACGGTCGACACGGGCATCGCAGCAGTACATGAAGCGAATGTAGCGAAGGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGAAGGTGGCGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCATTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGTGGTTTAAAACATATGCGTATGACGAGAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAATGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAGCGC
CGGCTGTACACCTTTTATTATCAGATGATCCAGAGGAAGCGAAGGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTTGAAAATAACTTCCCGCCAGAAGAAAACAAAGT
ATTAGTACTTGCAAAAGAAGGCTGGAACCTAGGGGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTACCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGCTTAAATGAACAAGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGTAAAGTAGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAAAAACTAGCTCCATTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTAAAAGATGCAGAGTTAGAAAGCATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCGTCCATTAATGAATGGAATAATTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGTGAG
CTATTTAAGGTAGGATTCCCAGCGCGAATTTATACGCTATTTTATCAAGAGAATAACCATTTATTTAGTACAGTTCCGAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.196

99.358

0.519

Multiple sequence alignment