Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EUC39_RS25910 Genome accession   NZ_CP040334
Coordinates   5187521..5191036 (-) Length   1171 a.a.
NCBI ID   WP_106822807.1    Uniprot ID   -
Organism   Bacillus cereus strain DLOU-Changhai     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5182521..5196036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUC39_RS25890 - 5182717..5183103 (+) 387 WP_106822801.1 spore germination protein GerPE -
  EUC39_RS25895 gerPF 5183146..5183361 (+) 216 WP_001141570.1 spore germination protein GerPF -
  EUC39_RS25900 - 5183497..5183775 (-) 279 WP_106822803.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EUC39_RS25905 addA 5183799..5187524 (-) 3726 WP_106822805.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EUC39_RS25910 addB 5187521..5191036 (-) 3516 WP_106822807.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EUC39_RS25915 lepB 5191153..5191716 (-) 564 WP_023521412.1 signal peptidase I -
  EUC39_RS25920 - 5191773..5192366 (-) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  EUC39_RS25925 - 5192673..5193254 (-) 582 WP_000444444.1 competence protein ComK -
  EUC39_RS25930 - 5193539..5193724 (+) 186 WP_106822809.1 hypothetical protein -
  EUC39_RS25935 - 5194068..5194271 (+) 204 WP_064532880.1 hypothetical protein -
  EUC39_RS25940 cspA 5194473..5194676 (-) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  EUC39_RS25945 - 5194961..5195455 (+) 495 WP_064532878.1 DUF6584 family protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134109.41 Da        Isoelectric Point: 5.1452

>NTDB_id=363327 EUC39_RS25910 WP_106822807.1 5187521..5191036(-) (addB) [Bacillus cereus strain DLOU-Changhai]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGAKVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEAVAREIRKLVADEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEVMREEMDEFENYCLAYGVQGKRWTAEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAEKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=363327 EUC39_RS25910 WP_106822807.1 5187521..5191036(-) (addB) [Bacillus cereus strain DLOU-Changhai]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAACGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTACAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATACGATGATTTTGAACGTGCTTTAATTGGAAAATACTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGACGGGTTTCATTCAT
TTTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAAAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGCTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGATGAAAAATATCGTTACCGAGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTACAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACACCACCCATTGGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGTTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGTGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTGCTGAAGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGCTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTGTTTTTAGAAGAACTGGATGTTCCGAAGAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACATTTGTAGAGATGCTTGGGGAAGAGAAA
ATGTCTCTTTCTATGTTTACTGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAATATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGAGGGTG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACGAATGACGTAAATGATTTATCACGTTCAGAACAAATTTCATATGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGGAGAAACTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAATCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCAGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCTATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATTGCGCCGTTATTACAACGACAAATTTTA
TTAAGCTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGTGGTACAGGATCACTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTCGGTCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493

Multiple sequence alignment