Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EUC39_RS10250 Genome accession   NZ_CP040334
Coordinates   2023850..2026189 (+) Length   779 a.a.
NCBI ID   WP_071729597.1    Uniprot ID   A0A9X5ZQR6
Organism   Bacillus cereus strain DLOU-Changhai     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2018850..2031189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUC39_RS10235 - 2019880..2020164 (+) 285 WP_000886979.1 post-transcriptional regulator -
  EUC39_RS10240 secDF 2020434..2022698 (+) 2265 WP_001119065.1 protein translocase subunit SecDF -
  EUC39_RS10245 - 2022855..2023748 (+) 894 WP_000409746.1 cation diffusion facilitator family transporter -
  EUC39_RS10250 recJ 2023850..2026189 (+) 2340 WP_071729597.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EUC39_RS10255 - 2026237..2026749 (+) 513 WP_000346217.1 adenine phosphoribosyltransferase -
  EUC39_RS10260 relA 2026960..2029143 (+) 2184 WP_001262795.1 GTP diphosphokinase -
  EUC39_RS10265 - 2029160..2029600 (+) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  EUC39_RS27250 - 2029766..2029942 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88137.64 Da        Isoelectric Point: 4.8429

>NTDB_id=363299 EUC39_RS10250 WP_071729597.1 2023850..2026189(+) (recJ) [Bacillus cereus strain DLOU-Changhai]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAIEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLEDYKEKLMHASEVTHLDDQYIVLLDLPSGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=363299 EUC39_RS10250 WP_071729597.1 2023850..2026189(+) (recJ) [Bacillus cereus strain DLOU-Changhai]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAACGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAACAAATATTAATATTTGGTGATTACGATGCAGACGGAGTAAGTAGTACAACTGTGTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTATATTCCAAATCGTTTTACAGAAGGGTATGGGCCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACTGTCGACACTGGCATCGCAGCAGTACATGAAGCGAATGTAGCAAAAGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGATGGCGGTGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTGTCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAAAAATATTGGTTTAAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAACGC
CGGCTGTACACCTTTTATTATCAGATGATCCTGAGGAAGCGAAAGAGTTGGCCGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTTGAAAATAACTTCCCACCAGAAGAAAACAAAGT
GTTAGTACTTGCAAAAGAAGGTTGGAATCCAGGTGTAATTGGGATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACTATTGTATTATGTATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCGCGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGGCATCCAATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGCTTAAATGAACAAGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGCAAAGTAGAGGATGTAACACTAGCGGCAATTGAAGATATGCAGAAGTTAGCACCATTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTGAAAGATGCAGAGTTAGAAAGTATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCATCCATCAATGAATGGAACAATTTTAAGAAGCCACAATTAATGGTACAA
GATATTGCTATAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTGCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGGACTATAAAGAAAAGCTTATGC
ATGCGTCAGAAGTAACGCATTTGGATGATCAATACATCGTGTTACTCGATTTGCCAAGCGGAACAGATGAATTACGTGAG
TTATTTAAAGTAGGATTCCCAGCGCGAATTTATACGCTGTTTTATCAAGAAAATAACCATCTATTTAGTACAGTTCCTAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGCCATAAAGGCTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.326

99.358

0.52

Multiple sequence alignment