Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FBB35_RS01735 Genome accession   NZ_CP040094
Coordinates   375607..376713 (-) Length   368 a.a.
NCBI ID   WP_174708221.1    Uniprot ID   A0A6N0LJY5
Organism   Nostoc sp. TCL240-02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370607..381713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBB35_RS01720 (FBB35_01710) - 371404..373827 (-) 2424 WP_174708218.1 type IV pilus secretin family protein -
  FBB35_RS01725 (FBB35_01715) - 373964..374740 (-) 777 WP_174708219.1 pilus assembly protein PilO -
  FBB35_RS01730 (FBB35_01720) - 374737..375513 (-) 777 WP_174708220.1 PilN domain-containing protein -
  FBB35_RS01735 (FBB35_01725) pilM 375607..376713 (-) 1107 WP_174708221.1 type IV pilus assembly protein PilM Machinery gene
  FBB35_RS01740 (FBB35_01730) - 376867..378165 (+) 1299 WP_174708222.1 sugar ABC transporter substrate-binding protein -
  FBB35_RS01745 (FBB35_01735) - 378224..379510 (-) 1287 WP_174708223.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  FBB35_RS01750 (FBB35_01740) - 379606..379731 (-) 126 WP_174708224.1 photosystem II protein Y -
  FBB35_RS01755 (FBB35_01745) - 379981..380508 (-) 528 WP_174708225.1 gamma carbonic anhydrase family protein -
  FBB35_RS01760 (FBB35_01755) - 380749..381261 (+) 513 WP_163939442.1 TIGR02652 family protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40691.65 Da        Isoelectric Point: 4.4583

>NTDB_id=362024 FBB35_RS01735 WP_174708221.1 375607..376713(-) (pilM) [Nostoc sp. TCL240-02]
MVKNFTRLFNKSNKGVGIELAPERVNVVQLRKQRQGLKLETFSSVAVPEGVVTDGQITDPAAMAQLIQQALAESKIKTTR
VATGVPGRDSIVRIIPVPTELDDKELREMVLNHEAGLYLPYPREEADVDYQKLDYFVDEDGIEKVHVLLVATRKEITDTY
INTFEQAGLQIDVLEINSFALIRTIREQLRQFGPQEAAVLVDIEFDSTEIAIIVNGVPQFSRTVPIGTYQIQTALARAMS
LPTSRDMELLHGMIIPPTPLDGGKTGVTEIDPGMAAILRVLGELTDELRRSIDFYLNQSENLEVAQILLAGPGGGLQQLD
EFFTQRLSLPTTQIDPIGSLSLEVDTEKYPQVQRSGLAIVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=362024 FBB35_RS01735 WP_174708221.1 375607..376713(-) (pilM) [Nostoc sp. TCL240-02]
GTGGTAAAGAACTTCACTCGTCTGTTTAACAAATCGAATAAAGGGGTCGGCATTGAACTTGCTCCCGAAAGAGTAAATGT
AGTTCAGCTACGCAAACAGCGTCAAGGCTTGAAACTAGAAACCTTTTCATCGGTAGCAGTTCCAGAAGGCGTAGTTACCG
ATGGTCAAATCACCGACCCCGCAGCAATGGCGCAATTAATCCAGCAGGCGCTAGCTGAAAGCAAAATCAAAACTACTCGT
GTTGCCACTGGTGTACCAGGGCGAGATTCCATCGTTCGGATTATACCTGTACCAACGGAGTTAGATGACAAAGAACTGCG
AGAAATGGTGCTAAACCATGAAGCAGGTTTGTATTTACCCTATCCCCGTGAAGAAGCTGATGTAGATTATCAGAAACTTG
ACTACTTTGTAGATGAGGATGGCATTGAAAAAGTACATGTACTTCTAGTAGCCACTCGCAAGGAAATAACGGATACCTAT
ATAAATACATTCGAACAGGCAGGACTACAAATTGATGTTTTAGAAATTAACAGTTTTGCCCTGATTCGGACTATTCGTGA
GCAACTGCGACAATTTGGGCCGCAAGAAGCAGCAGTCCTAGTTGATATAGAGTTCGACAGTACAGAAATCGCCATCATCG
TTAACGGAGTCCCGCAATTTTCGCGCACAGTGCCCATAGGTACTTATCAAATCCAAACTGCGTTAGCAAGGGCAATGAGC
TTACCCACATCACGAGATATGGAACTGTTACACGGAATGATTATTCCCCCAACTCCCCTAGATGGTGGGAAAACCGGTGT
TACCGAAATCGATCCTGGTATGGCAGCCATATTAAGAGTATTGGGAGAACTAACGGATGAATTGCGCCGTTCGATCGATT
TTTACCTTAATCAAAGTGAAAATTTGGAGGTAGCGCAGATTTTATTAGCTGGGCCAGGAGGCGGACTCCAACAGCTAGAT
GAATTCTTTACCCAACGATTGAGCTTGCCAACTACCCAAATAGATCCAATCGGGTCTTTGTCGTTAGAAGTTGACACTGA
GAAATATCCACAGGTACAACGCTCTGGCTTGGCGATTGTACTTGGTCTAGGTATGCGGGAGGTGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-238)

Type IV pilus assembly protein PilM; (PilM_2)

(283-367)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0LJY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

65.668

99.728

0.655

Multiple sequence alignment