Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GVI77_RS01235 Genome accession   NZ_CP047710
Coordinates   273381..274145 (-) Length   254 a.a.
NCBI ID   WP_001136234.1    Uniprot ID   -
Organism   Escherichia coli strain Eco-15     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 268381..279145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI77_RS01205 (GVI77_01205) - 269015..270373 (-) 1359 WP_001545659.1 PTS galactitol transporter subunit IIC -
  GVI77_RS01210 (GVI77_01210) - 270450..270731 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  GVI77_RS01215 (GVI77_01215) - 270728..271201 (-) 474 WP_001161648.1 PTS sugar transporter subunit IIA -
  GVI77_RS01220 (GVI77_01220) - 271226..271972 (-) 747 WP_001296489.1 UTRA domain-containing protein -
  GVI77_RS01225 (GVI77_01225) nikR 272171..272572 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  GVI77_RS01230 (GVI77_01230) nikE 272578..273384 (-) 807 WP_000173682.1 nickel import ATP-binding protein NikE -
  GVI77_RS01235 (GVI77_01235) amiE 273381..274145 (-) 765 WP_001136234.1 nickel import ATP-binding protein NikD Regulator
  GVI77_RS01240 (GVI77_01240) nikC 274145..274978 (-) 834 WP_001008957.1 nickel ABC transporter permease subunit NikC -
  GVI77_RS01245 (GVI77_01245) nikB 274975..275919 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GVI77_RS01250 (GVI77_01250) nikA 275919..277493 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  GVI77_RS01255 (GVI77_01255) acpT 277604..278191 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26811.42 Da        Isoelectric Point: 6.9014

>NTDB_id=360922 GVI77_RS01235 WP_001136234.1 273381..274145(-) (amiE) [Escherichia coli strain Eco-15]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGNVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=360922 GVI77_RS01235 WP_001136234.1 273381..274145(-) (amiE) [Escherichia coli strain Eco-15]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAAGCACGCATCCTCGATCTACTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTAGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAAATTGTCGAACAGGGCAATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398