Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GUU84_RS20630 Genome accession   NZ_CP047662
Coordinates   4317863..4318627 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain LD93-1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4312863..4323627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUU84_RS20610 (GUU84_20610) acpT 4313817..4314404 (+) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -
  GUU84_RS20615 (GUU84_20615) nikA 4314515..4316089 (+) 1575 WP_111738580.1 nickel ABC transporter substrate-binding protein -
  GUU84_RS20620 (GUU84_20620) nikB 4316089..4317033 (+) 945 WP_160188268.1 nickel ABC transporter permease subunit NikB -
  GUU84_RS20625 (GUU84_20625) nikC 4317030..4317863 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GUU84_RS20630 (GUU84_20630) amiE 4317863..4318627 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  GUU84_RS20635 (GUU84_20635) nikE 4318624..4319430 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  GUU84_RS20640 (GUU84_20640) nikR 4319436..4319837 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=360721 GUU84_RS20630 WP_001136236.1 4317863..4318627(+) (amiE) [Escherichia coli strain LD93-1]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=360721 GUU84_RS20630 WP_001136236.1 4317863..4318627(+) (amiE) [Escherichia coli strain LD93-1]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398