Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GUK18_RS13180 Genome accession   NZ_CP047600
Coordinates   2711254..2712018 (+) Length   254 a.a.
NCBI ID   WP_024223028.1    Uniprot ID   -
Organism   Escherichia coli O84:H7 strain Trh52     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2706254..2717018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUK18_RS13160 (GUK18_13190) acpT 2707208..2707795 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  GUK18_RS13165 (GUK18_13195) nikA 2707906..2709480 (+) 1575 WP_021580344.1 nickel ABC transporter substrate-binding protein -
  GUK18_RS13170 (GUK18_13200) nikB 2709480..2710424 (+) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  GUK18_RS13175 (GUK18_13205) nikC 2710421..2711254 (+) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  GUK18_RS13180 (GUK18_13210) amiE 2711254..2712018 (+) 765 WP_024223028.1 nickel import ATP-binding protein NikD Regulator
  GUK18_RS13185 (GUK18_13215) nikE 2712015..2712821 (+) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  GUK18_RS13190 (GUK18_13220) nikR 2712827..2713228 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  GUK18_RS13195 (GUK18_13225) yhhJ 2713237..2714361 (-) 1125 WP_001296492.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26754.37 Da        Isoelectric Point: 6.9014

>NTDB_id=360345 GUK18_RS13180 WP_024223028.1 2711254..2712018(+) (amiE) [Escherichia coli O84:H7 strain Trh52]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADGATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=360345 GUK18_RS13180 WP_024223028.1 2711254..2712018(+) (amiE) [Escherichia coli O84:H7 strain Trh52]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGGCGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTGGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398