Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   I33_RS13545 Genome accession   NC_017195
Coordinates   2722796..2725153 (-) Length   785 a.a.
NCBI ID   WP_014477595.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. RO-NN-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2717796..2730153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I33_RS13520 (I33_2908) etfB 2718238..2719011 (-) 774 WP_014477591.1 electron transfer flavoprotein subunit beta -
  I33_RS13525 (I33_2909) fadB 2719026..2719802 (-) 777 WP_014477592.1 enoyl-CoA hydratase -
  I33_RS13530 (I33_2910) fadR 2719817..2720401 (-) 585 WP_014477593.1 fatty acid metabolism transcriptional regulator FadR -
  I33_RS13535 (I33_2911) lcfA 2720506..2722188 (-) 1683 WP_014477594.1 long-chain-fatty-acid--CoA ligase LcfA -
  I33_RS13540 (I33_2912) yshE 2722377..2722781 (-) 405 WP_003237674.1 DUF350 domain-containing protein -
  I33_RS13545 (I33_2913) mutS/mutS2 2722796..2725153 (-) 2358 WP_014477595.1 endonuclease MutS2 Machinery gene
  I33_RS13550 (I33_2914) polX 2725174..2726886 (-) 1713 WP_014477596.1 DNA polymerase/3'-5' exonuclease PolX -
  I33_RS13555 (I33_2915) yshB 2726960..2727493 (-) 534 WP_014477597.1 CvpA family protein -
  I33_RS13560 (I33_2916) zapA 2727500..2727757 (-) 258 WP_003229534.1 cell division protein ZapA -
  I33_RS13565 (I33_2917) rnhC 2727891..2728829 (+) 939 WP_014477598.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87370.71 Da        Isoelectric Point: 6.1364

>NTDB_id=36022 I33_RS13545 WP_014477595.1 2722796..2725153(-) (mutS/mutS2) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEYTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPEQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEEAEQQAAEKVKAAMKEAEDIIHELRSIKEEHKSFKDHELINAKKRLEGAVPTFEKSKKPEKTKAQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEPKKEKIITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=36022 I33_RS13545 WP_014477595.1 2722796..2725153(-) (mutS/mutS2) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCCTCTGCTTCTATAGACGAAATCAAAAAACAGCTGGATGAGGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGCGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
CCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATCGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACATTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCATCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACACTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAACAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGTATACAGAGGAGCTATT
TCTAGATTTGCAAGTGCTGCAGACGCTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATTCGTTTAAAAAAAGCCCGCCATCCATTGCTACCGCCTGAACAGGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACCATTGTTATCACAGGGCCAAACACCGGGGGGAAAACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAGTCAGGTCTGCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGCGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCACATGGTGAACATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGCCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATTTTGGATGACGTGCACCGCACCAATGCACGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCAAGCGTTGAATTTGACATCGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGGCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTTCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAGCAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAAACAGAATCAATCAGAAAAGAAGCGGAAAAACTCCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGAGGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATGAAAGAAGC
CGAGGACATTATTCATGAATTGCGCTCCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATCAACGCGAAGA
AACGGTTAGAAGGCGCAGTTCCTACCTTTGAAAAGTCCAAAAAACCGGAAAAAACGAAAGCGCAAAAACGCGACTTTAAG
CCTGGCGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACACTGCTTGAAAAAACAGGCGGCAATGAATGGAATGT
TCAAATCGGCATATTAAAAATGAAAGTAAAAGAAAAAGATCTGGAGTTTATCAAATCAGCTCCGGAGCCAAAAAAAGAAA
AAATCATTACAGCGGTCAAGGGAAAGGACTATCACGTATCGCTTGAACTTGATCTTCGCGGCGAACGCTATGAAAATGCC
CTCAGCCGTGTTGAAAAATACTTGGATGATGCAGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCACCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.108

100

0.991

Multiple sequence alignment