Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   E8M05_RS03910 Genome accession   NZ_CP039457
Coordinates   716003..716869 (+) Length   288 a.a.
NCBI ID   WP_003064105.1    Uniprot ID   E0PCS6
Organism   Streptococcus pasteurianus strain WUSP067     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 711003..721869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M05_RS03880 (E8M05_03875) - 711088..711966 (+) 879 WP_003064089.1 F0F1 ATP synthase subunit gamma -
  E8M05_RS03885 (E8M05_03880) atpD 712033..713439 (+) 1407 WP_003064092.1 F0F1 ATP synthase subunit beta -
  E8M05_RS03890 (E8M05_03885) - 713452..713868 (+) 417 WP_003064095.1 F0F1 ATP synthase subunit epsilon -
  E8M05_RS03895 (E8M05_03890) - 714124..714360 (+) 237 WP_003064099.1 DUF1146 family protein -
  E8M05_RS03900 (E8M05_03895) murA 714423..715697 (+) 1275 WP_041974287.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  E8M05_RS03905 (E8M05_03900) - 715700..715888 (+) 189 WP_003064103.1 DNA-directed RNA polymerase subunit beta -
  E8M05_RS03910 (E8M05_03905) endA 716003..716869 (+) 867 WP_003064105.1 DNA/RNA non-specific endonuclease Machinery gene
  E8M05_RS03915 (E8M05_03910) pheS 717156..718199 (+) 1044 WP_013642932.1 phenylalanine--tRNA ligase subunit alpha -
  E8M05_RS03920 (E8M05_03915) - 718235..718727 (+) 493 Protein_699 GNAT family N-acetyltransferase -
  E8M05_RS03925 (E8M05_03920) pheT 718798..721203 (+) 2406 WP_003064115.1 phenylalanine--tRNA ligase subunit beta -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 30866.05 Da        Isoelectric Point: 6.4909

>NTDB_id=358790 E8M05_RS03910 WP_003064105.1 716003..716869(+) (endA) [Streptococcus pasteurianus strain WUSP067]
MAKKSKLSKQIKSLLSLVILLVGIGTGWVTISDSNDPLNQVVSLITGDSSSEITSNQSGSDSATPTQALAETVLTDSVKE
QLGTNIEWNGAGAYIINNNQTSLNAKVSSQPYANNKTKTVQGQTVPTVANALLSKSTRQYKSREETGNGSTSWKPAGWHQ
VQDLSGTYSHAVDRGHLLGYALVGGLKGFDASTSNPDNVATQTAWANEANSESSTGQNYYECLVRKALDQNKRVRYRVTL
IYDGDNLLASGTHLEAKSSDGSLEFNVFIPNVQSGLTFDYYSGQVSVN

Nucleotide


Download         Length: 867 bp        

>NTDB_id=358790 E8M05_RS03910 WP_003064105.1 716003..716869(+) (endA) [Streptococcus pasteurianus strain WUSP067]
ATGGCAAAGAAATCAAAATTGTCTAAACAGATAAAATCCCTGCTGTCTTTGGTGATTTTGTTAGTTGGGATTGGTACTGG
TTGGGTGACGATATCAGATTCGAACGACCCGCTCAATCAAGTCGTTTCGTTAATTACTGGTGATTCCTCTTCTGAAATAA
CATCAAATCAATCGGGTTCTGATTCAGCAACGCCAACACAAGCTTTAGCAGAGACGGTATTAACAGATAGTGTCAAAGAG
CAGTTGGGAACAAATATAGAATGGAATGGTGCGGGAGCCTATATTATCAATAATAATCAAACCTCTCTAAATGCAAAAGT
TTCAAGTCAACCTTACGCCAACAACAAAACGAAAACGGTGCAAGGTCAGACCGTTCCAACGGTTGCTAATGCTTTGCTAA
GTAAGTCAACTCGTCAATACAAAAGTCGTGAGGAGACTGGAAATGGCTCAACGTCTTGGAAGCCAGCTGGTTGGCATCAA
GTTCAAGACCTTTCAGGGACTTATAGCCATGCGGTAGATCGTGGTCATTTGCTTGGCTATGCTTTGGTTGGTGGTCTAAA
AGGCTTTGATGCGTCAACAAGCAATCCTGACAATGTGGCAACACAGACTGCCTGGGCAAACGAGGCAAATTCGGAAAGTT
CAACTGGTCAAAATTACTATGAATGCCTTGTCCGCAAAGCTCTTGACCAAAATAAACGAGTTCGTTACCGTGTTACCTTG
ATTTACGATGGAGACAATCTTTTAGCAAGTGGCACGCATTTGGAAGCCAAATCATCCGATGGTAGTCTAGAATTTAACGT
CTTTATTCCAAATGTGCAAAGCGGACTAACATTTGATTATTATTCAGGTCAAGTTAGTGTGAATTGA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(96-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0PCS6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

65.726

86.111

0.566
  endA Streptococcus pneumoniae D39

65.726

86.111

0.566
  endA Streptococcus pneumoniae R6

65.726

86.111

0.566
  endA Streptococcus pneumoniae TIGR4

65.726

86.111

0.566

Multiple sequence alignment