Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TNAP_RS01820 Genome accession   NC_013642
Coordinates   355533..356417 (-) Length   294 a.a.
NCBI ID   WP_012895940.1    Uniprot ID   D2C668
Organism   Thermotoga petrophila RKU-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350533..361417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TNAP_RS01790 (Tnap_0346) - 351464..351943 (+) 480 WP_081440579.1 flavin reductase family protein -
  TNAP_RS01795 (Tnap_0347) sfsA 351940..352608 (+) 669 WP_012895938.1 DNA/RNA nuclease SfsA -
  TNAP_RS01800 (Tnap_0348) - 352612..352806 (+) 195 WP_004081301.1 hypothetical protein -
  TNAP_RS01805 (Tnap_0349) - 352825..353238 (-) 414 WP_004081299.1 Rrf2 family transcriptional regulator -
  TNAP_RS01810 (Tnap_0350) - 353240..354904 (-) 1665 WP_012895939.1 peptidoglycan DD-metalloendopeptidase family protein -
  TNAP_RS01815 (Tnap_0351) - 354880..355536 (-) 657 WP_011943013.1 DUF2225 domain-containing protein -
  TNAP_RS01820 (Tnap_0352) pilA 355533..356417 (-) 885 WP_012895940.1 signal recognition particle-docking protein FtsY Machinery gene
  TNAP_RS01825 (Tnap_0353) - 356531..357910 (+) 1380 WP_004081291.1 DegQ family serine endoprotease -
  TNAP_RS01830 (Tnap_0354) - 358007..359158 (+) 1152 WP_012895941.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  TNAP_RS01835 (Tnap_0355) lnt 359151..360614 (+) 1464 WP_011943009.1 apolipoprotein N-acyltransferase -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32046.26 Da        Isoelectric Point: 5.3215

>NTDB_id=35853 TNAP_RS01820 WP_012895940.1 355533..356417(-) (pilA) [Thermotoga petrophila RKU-10]
MGLFDFLKKGLQKTKETFFGRVVKLLKGKKLDDETREELEELLIQADVGVETTEYILERLEEKDGDALESLKEIILEILN
FDTKLNVPPEPPFVIMVVGVNGTGKTTSCGKLAKMFVDEGKSVVLAAADTFRAAAIEQLKIWGERVGATVISHSEGADPA
AVAFDAVAHALARNKDVVIIDTAGRLHTKKNLMEELRKVHRVVKKKIPDAPHETLLVIDATTGQNGLVQAKIFKEAVNVT
GIILTKLDGTAKGGITLAIARELGIPIKFIGVGEKAEDLRPFDPEAFVEVLISE

Nucleotide


Download         Length: 885 bp        

>NTDB_id=35853 TNAP_RS01820 WP_012895940.1 355533..356417(-) (pilA) [Thermotoga petrophila RKU-10]
ATGGGACTCTTCGATTTTTTGAAAAAGGGACTCCAGAAGACGAAAGAAACGTTTTTTGGACGAGTGGTGAAGCTCCTCAA
AGGAAAAAAGCTGGACGATGAAACCCGCGAGGAGCTGGAAGAACTTCTCATTCAAGCGGACGTGGGAGTCGAGACAACGG
AATACATTCTGGAGAGATTGGAAGAGAAGGACGGAGACGCTCTCGAGTCTCTCAAGGAAATCATTCTGGAAATTCTGAAC
TTCGATACGAAGCTGAACGTTCCTCCTGAACCCCCGTTCGTGATCATGGTTGTTGGTGTCAACGGAACGGGAAAGACCAC
CTCCTGTGGAAAGCTCGCAAAGATGTTCGTGGATGAAGGAAAGAGTGTGGTGCTCGCTGCGGCGGATACTTTCAGAGCGG
CCGCCATAGAACAGCTGAAGATATGGGGAGAACGTGTCGGTGCCACGGTGATATCCCACTCGGAAGGAGCAGATCCGGCC
GCCGTTGCCTTTGACGCGGTGGCTCACGCTCTCGCCAGAAACAAAGATGTGGTCATAATAGACACCGCCGGAAGACTTCA
CACGAAGAAGAATCTCATGGAGGAATTGCGGAAGGTTCACAGAGTTGTCAAAAAGAAGATCCCCGACGCTCCGCACGAAA
CGCTCCTCGTGATAGACGCCACCACCGGCCAAAACGGACTTGTTCAGGCAAAAATATTCAAAGAAGCAGTGAATGTCACC
GGTATAATTCTCACAAAGCTCGACGGAACCGCGAAAGGCGGAATAACTCTGGCCATTGCCCGGGAACTGGGAATACCCAT
AAAGTTCATCGGTGTCGGAGAAAAGGCAGAAGACCTGAGGCCTTTCGATCCTGAAGCCTTTGTGGAGGTATTGATTTCAG
AATGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(92-292)

SRP54-type protein, helical bundle domain (SRP54_N)

(22-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2C668

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50

100

0.514

Multiple sequence alignment