Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GRF62_RS10100 Genome accession   NZ_CP047127
Coordinates   2135852..2136616 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli K-12     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2130852..2141616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRF62_RS10090 (GRF62_10090) nikR 2134642..2135043 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  GRF62_RS10095 (GRF62_10095) nikE 2135049..2135855 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  GRF62_RS10100 (GRF62_10100) amiE 2135852..2136616 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  GRF62_RS10105 (GRF62_10105) nikC 2136616..2137449 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GRF62_RS10110 (GRF62_10110) nikB 2137446..2138390 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GRF62_RS10115 (GRF62_10115) nikA 2138390..2139964 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  GRF62_RS10120 (GRF62_10120) acpT 2140075..2140662 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=357403 GRF62_RS10100 WP_001136229.1 2135852..2136616(-) (amiE) [Escherichia coli K-12]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=357403 GRF62_RS10100 WP_001136229.1 2135852..2136616(-) (amiE) [Escherichia coli K-12]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398