Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GQF95_RS20365 Genome accession   NZ_CP047010
Coordinates   4177977..4178741 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain 19-5     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4172977..4183741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQF95_RS20345 (GQF95_20325) acpT 4173931..4174518 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  GQF95_RS20350 (GQF95_20330) nikA 4174629..4176203 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  GQF95_RS20355 (GQF95_20335) nikB 4176203..4177147 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GQF95_RS20360 (GQF95_20340) nikC 4177144..4177977 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GQF95_RS20365 (GQF95_20345) amiE 4177977..4178741 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  GQF95_RS20370 (GQF95_20350) nikE 4178738..4179544 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  GQF95_RS20375 (GQF95_20355) nikR 4179550..4179951 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=356889 GQF95_RS20365 WP_001136229.1 4177977..4178741(+) (amiE) [Escherichia coli strain 19-5]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=356889 GQF95_RS20365 WP_001136229.1 4177977..4178741(+) (amiE) [Escherichia coli strain 19-5]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCTCCCAAACATACAGTGACACGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398