Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GQF94_RS13360 Genome accession   NZ_CP047002
Coordinates   2756541..2757305 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain J-8     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2751541..2762305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GQF94_RS13340 (GQF94_13325) acpT 2752495..2753082 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  GQF94_RS13345 (GQF94_13330) nikA 2753193..2754767 (+) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  GQF94_RS13350 (GQF94_13335) nikB 2754767..2755711 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GQF94_RS13355 (GQF94_13340) nikC 2755708..2756541 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GQF94_RS13360 (GQF94_13345) amiE 2756541..2757305 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  GQF94_RS13365 (GQF94_13350) nikE 2757302..2758108 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  GQF94_RS13370 (GQF94_13355) nikR 2758114..2758515 (+) 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
  GQF94_RS13375 (GQF94_13360) - 2758635..2758994 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  GQF94_RS13380 (GQF94_13365) - 2758991..2759266 (-) 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin -
  GQF94_RS13385 (GQF94_13370) yhhJ 2759339..2760463 (-) 1125 WP_001615305.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=356787 GQF94_RS13360 WP_001136236.1 2756541..2757305(+) (amiE) [Escherichia coli strain J-8]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=356787 GQF94_RS13360 WP_001136236.1 2756541..2757305(+) (amiE) [Escherichia coli strain J-8]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment