Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   E5135_RS05370 Genome accession   NZ_CP038873
Coordinates   1171217..1171762 (-) Length   181 a.a.
NCBI ID   WP_061406243.1    Uniprot ID   -
Organism   Pasteurella multocida strain FCf45     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1166217..1176762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5135_RS05345 (E5135_05350) - 1166428..1166865 (-) 438 WP_005725139.1 isoprenylcysteine carboxylmethyltransferase family protein -
  E5135_RS05350 (E5135_05355) - 1167077..1167688 (-) 612 WP_005725137.1 YfgM family protein -
  E5135_RS05355 (E5135_05360) hisS 1167707..1168978 (-) 1272 WP_061406242.1 histidine--tRNA ligase -
  E5135_RS05360 (E5135_05365) ispG 1169008..1170111 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E5135_RS05365 (E5135_05370) - 1170138..1171094 (-) 957 WP_005725132.1 RodZ family helix-turn-helix domain-containing protein -
  E5135_RS05370 (E5135_05375) pilF/pilF2 1171217..1171762 (-) 546 WP_061406243.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E5135_RS05375 (E5135_05380) - 1171801..1172985 (-) 1185 WP_061406244.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  E5135_RS05380 (E5135_05385) - 1173165..1174418 (-) 1254 WP_005755688.1 AI-2E family transporter -
  E5135_RS05385 (E5135_05390) glnB 1174419..1174757 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  E5135_RS05390 (E5135_05395) mog 1174802..1175380 (-) 579 WP_005725110.1 molybdopterin adenylyltransferase -
  E5135_RS05395 (E5135_05400) - 1175406..1176335 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20867.39 Da        Isoelectric Point: 7.8085

>NTDB_id=356131 E5135_RS05370 WP_061406243.1 1171217..1171762(-) (pilF/pilF2) [Pasteurella multocida strain FCf45]
MNRTFRFGIASIFSLLLCACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQYLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=356131 E5135_RS05370 WP_061406243.1 1171217..1171762(-) (pilF/pilF2) [Pasteurella multocida strain FCf45]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGATGCTGTCCGTTATCAACAA
AATTTGCAATATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

45.405

100

0.464

Multiple sequence alignment