Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY55_RS09960 Genome accession   NZ_CP046831
Coordinates   188168..189892 (+) Length   574 a.a.
NCBI ID   WP_031847361.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2012AW-0224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 183168..194892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY55_RS09935 gspM 183573..184223 (+) 651 WP_005497276.1 type II secretion system protein GspM -
  GPY55_RS09940 - 184216..184545 (+) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  GPY55_RS09945 mshL 184566..186209 (+) 1644 WP_020841334.1 pilus (MSHA type) biogenesis protein MshL -
  GPY55_RS09950 - 186253..187098 (+) 846 WP_005456312.1 AAA family ATPase -
  GPY55_RS09955 - 187095..188171 (+) 1077 WP_025541499.1 tetratricopeptide repeat protein -
  GPY55_RS09960 pilB 188168..189892 (+) 1725 WP_031847361.1 GspE/PulE family protein Machinery gene
  GPY55_RS09965 - 189901..191124 (+) 1224 WP_021484749.1 type II secretion system F family protein -
  GPY55_RS09970 - 191128..191589 (+) 462 WP_024701144.1 hypothetical protein -
  GPY55_RS09975 - 191884..192402 (+) 519 WP_031847360.1 type II secretion system protein -
  GPY55_RS09980 - 192466..192699 (+) 234 WP_005388453.1 MSHA biogenesis protein MshA -
  GPY55_RS09985 - 192815..193294 (+) 480 WP_031847359.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY55_RS09990 - 193291..193890 (+) 600 WP_025542026.1 type II secretion system protein -
  GPY55_RS09995 - 193890..194603 (+) 714 WP_031847358.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63944.25 Da        Isoelectric Point: 5.1528

>NTDB_id=356039 GPY55_RS09960 WP_031847361.1 188168..189892(+) (pilB) [Vibrio parahaemolyticus strain 2012AW-0224]
MKIQLRKRLGDLLVEEDIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=356039 GPY55_RS09960 WP_031847361.1 188168..189892(+) (pilB) [Vibrio parahaemolyticus strain 2012AW-0224]
ATGAAAATTCAGTTGAGAAAACGTTTAGGTGACCTACTCGTTGAAGAAGACATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTTTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGTGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACTCAAGAGTCTTTAATGAACTTGCTCGGTGAGTACAACCTTGAGTTCATCATTGCGTCTGAGC
GTCAGTTGATCAGTAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCTTTTGCTGAGCAGTTACAAGCCGAG
CACCAAGACGTACAAAGCTTTGACTATGGTATCGATGAAGCTGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGCTTACGCCAGC
GTGTCGACGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGCCTGCTTAACCAGTCGTCAGGCTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACCGGCCCTACGGGGTCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGATCAAGAGACGGTAGAG
ATTGGCTTACGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCATACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACTCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAATTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

97.387

0.376