Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY25_RS00635 Genome accession   NZ_CP046779
Coordinates   114345..116069 (-) Length   574 a.a.
NCBI ID   WP_005497280.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2014V-1066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 109345..121069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY25_RS00600 - 109684..110397 (-) 714 WP_005497288.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY25_RS00605 - 110397..110996 (-) 600 WP_005497286.1 type II secretion system protein -
  GPY25_RS00610 - 110993..111472 (-) 480 WP_023650351.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY25_RS00615 - 111588..111821 (-) 234 WP_005388453.1 MSHA biogenesis protein MshA -
  GPY25_RS00620 - 111892..112353 (-) 462 WP_005497282.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GPY25_RS00625 - 112648..113109 (-) 462 WP_005456302.1 MSHA biogenesis protein MshF -
  GPY25_RS00630 - 113113..114336 (-) 1224 WP_005497281.1 type II secretion system F family protein -
  GPY25_RS00635 pilB 114345..116069 (-) 1725 WP_005497280.1 GspE/PulE family protein Machinery gene
  GPY25_RS00640 - 116066..117142 (-) 1077 WP_005497279.1 tetratricopeptide repeat protein -
  GPY25_RS00645 - 117139..117984 (-) 846 WP_005497278.1 AAA family ATPase -
  GPY25_RS00650 mshL 118028..119671 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  GPY25_RS00655 - 119692..120021 (-) 330 WP_020841335.1 MSHA biogenesis protein MshK -
  GPY25_RS00660 gspM 120014..120664 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63914.23 Da        Isoelectric Point: 5.2324

>NTDB_id=355424 GPY25_RS00635 WP_005497280.1 114345..116069(-) (pilB) [Vibrio parahaemolyticus strain 2014V-1066]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHRGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=355424 GPY25_RS00635 WP_005497280.1 114345..116069(-) (pilB) [Vibrio parahaemolyticus strain 2014V-1066]
ATGAAAATTCAGTTGAGAAAACGTTTAGGTGACCTACTCGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTTTTGTCTCAGCAACTAGGATTACCGTTGATCGATCTTGGACGTGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCAGAG
CACCAAGACGTACAAAGCTTTGACTATGGCATCGATGAAGCCGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGATGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCGCAAGATGGTCGCTTCAACATCAAAGTTCGTGGCCAGTCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGGTCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGATCAAGAGACGGTAGAG
ATTGGCTTACGAGCAGCATTAACTGGCCACTTAGTATTGAGTACGTTGCATACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTTCACAGAGGAGCAGGTTGTCAAAACTGTAACTTGACGGGATATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGCGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.998

97.387

0.38

Multiple sequence alignment