Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   GNT44_RS27675 Genome accession   NZ_CP046402
Coordinates   5837109..5837534 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain SE5331     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5832109..5842534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNT44_RS27660 (GNT44_27640) pilX 5832673..5833260 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  GNT44_RS27665 (GNT44_27645) pilY1 5833272..5836763 (+) 3492 WP_003094704.1 type 4a pilus biogenesis protein PilY1 -
  GNT44_RS27670 (GNT44_27650) pilY2 5836765..5837112 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  GNT44_RS27675 (GNT44_27655) comF 5837109..5837534 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  GNT44_RS27680 (GNT44_27660) ispH 5837581..5838525 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  GNT44_RS27685 (GNT44_27665) fkpB 5838611..5839051 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  GNT44_RS27690 (GNT44_27670) lspA 5839044..5839553 (-) 510 WP_003094728.1 signal peptidase II -
  GNT44_RS27695 (GNT44_27675) ileS 5839546..5842377 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=351698 GNT44_RS27675 WP_003094721.1 5837109..5837534(+) (comF) [Pseudomonas aeruginosa strain SE5331]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=351698 GNT44_RS27675 WP_003094721.1 5837109..5837534(+) (comF) [Pseudomonas aeruginosa strain SE5331]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383