Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GJD92_RS02115 Genome accession   NZ_CP046000
Coordinates   305834..306598 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain 1916D18     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 300834..311598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD92_RS02085 (GJD92_02085) - 301468..302826 (-) 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
  GJD92_RS02090 (GJD92_02090) - 302903..303184 (-) 282 WP_000084022.1 PTS sugar transporter subunit IIB -
  GJD92_RS02095 (GJD92_02095) - 303181..303654 (-) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  GJD92_RS02100 (GJD92_02100) - 303679..304425 (-) 747 WP_000839066.1 GntR family transcriptional regulator -
  GJD92_RS02105 (GJD92_02105) nikR 304624..305025 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  GJD92_RS02110 (GJD92_02110) nikE 305031..305837 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  GJD92_RS02115 (GJD92_02115) amiE 305834..306598 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  GJD92_RS02120 (GJD92_02120) nikC 306598..307431 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GJD92_RS02125 (GJD92_02125) nikB 307428..308372 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GJD92_RS02130 (GJD92_02130) nikA 308372..309946 (-) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  GJD92_RS02135 (GJD92_02135) acpT 310057..310644 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=349271 GJD92_RS02115 WP_001136236.1 305834..306598(-) (amiE) [Escherichia coli strain 1916D18]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=349271 GJD92_RS02115 WP_001136236.1 305834..306598(-) (amiE) [Escherichia coli strain 1916D18]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398