Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EXZ61_RS01970 Genome accession   NZ_CP036282
Coordinates   415901..417499 (+) Length   532 a.a.
NCBI ID   WP_142808509.1    Uniprot ID   -
Organism   Rhodoferax aquaticus strain Gr-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410901..422499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXZ61_RS01945 (EXZ61_01940) argS 411223..412932 (+) 1710 WP_142808500.1 arginine--tRNA ligase -
  EXZ61_RS01950 (EXZ61_01945) - 412956..413600 (+) 645 WP_237219060.1 SPOR domain-containing protein -
  EXZ61_RS01955 (EXZ61_01950) - 413659..414312 (+) 654 WP_142808504.1 thiol:disulfide interchange protein DsbA/DsbL -
  EXZ61_RS01960 (EXZ61_01955) lptA 414404..415021 (+) 618 WP_142808506.1 lipopolysaccharide transport periplasmic protein LptA -
  EXZ61_RS01965 (EXZ61_01960) lptB 415018..415785 (+) 768 WP_142808507.1 LPS export ABC transporter ATP-binding protein -
  EXZ61_RS01970 (EXZ61_01965) rpoN/rpoN1 415901..417499 (+) 1599 WP_142808509.1 RNA polymerase factor sigma-54 Machinery gene
  EXZ61_RS01975 (EXZ61_01970) - 417496..418761 (+) 1266 WP_142808510.1 class I SAM-dependent RNA methyltransferase -
  EXZ61_RS01980 (EXZ61_01975) - 418758..419177 (+) 420 WP_142808512.1 putative toxin-antitoxin system toxin component, PIN family -
  EXZ61_RS01985 (EXZ61_01980) - 419264..420016 (+) 753 WP_237219061.1 transporter substrate-binding domain-containing protein -
  EXZ61_RS01990 (EXZ61_01985) - 420047..421675 (-) 1629 WP_142808514.1 FMN-binding glutamate synthase family protein -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58784.39 Da        Isoelectric Point: 4.8434

>NTDB_id=347845 EXZ61_RS01970 WP_142808509.1 415901..417499(+) (rpoN/rpoN1) [Rhodoferax aquaticus strain Gr-4]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLELTQDEAVREDFGIAQADTPVTRDDREAETAPT
SIADYASTTSAEATNDDEATPLSADDGVSWEGDGTTDMAPDDGEWGGEAKASTNNLGDDGDFDATELARTQESLQAHLHR
QALRLRLSPTDTAALRFLIESLNDDGYLDEPLEDLARGLCGGDLDQLDDLVHHFTVALHLLHSLEPVGVGARNLGECLRL
QLKALQADEDLSDERSALLAVAVRMCSQPMDLLARRDIKRLVQLCDATDAQVREAIALIARLEPKPGRRFVDVQRNIVVP
DVLVVPVSKRPGVVNTKFRVLLNPEVMPRLRVHDIYANVLRNHRGGKDTASHAALQQRLQEARWFIKNIQQRFDTILRVS
TAIVERQSSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTFELKYFFGSGLGTESGGNASSTAVRALI
KQFISAESNLKPLSDNQISDMLKEQGIECARRTVAKYREGLKIAPASLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=347845 EXZ61_RS01970 WP_142808509.1 415901..417499(+) (rpoN/rpoN1) [Rhodoferax aquaticus strain Gr-4]
ATGAAGCAAGGGCTTTCCCTACGCGTCTCGCAACACCTAGCGCTGACGCCGCAACTGCAGCAATCTATTCGCCTCTTGCA
GCTCTCTACCTTGGAGCTAAGCCAAGAAGTGGAGCAGATGCTGGATGAAAATCCGTTTCTGGAGCTCACCCAAGACGAGG
CTGTGCGTGAAGACTTTGGCATTGCCCAGGCTGATACGCCGGTGACGCGCGACGACCGGGAAGCCGAAACTGCTCCTACT
TCTATAGCTGACTACGCAAGTACCACGAGCGCTGAGGCCACCAATGACGATGAAGCCACCCCGCTGTCTGCCGATGATGG
CGTGAGTTGGGAGGGCGATGGCACCACCGACATGGCGCCGGACGACGGCGAATGGGGTGGTGAAGCCAAGGCCAGTACCA
ACAACCTTGGTGATGATGGTGACTTTGACGCCACGGAGTTGGCGCGCACCCAAGAGTCTTTACAAGCCCATTTGCACCGC
CAAGCGCTGCGCTTGCGCCTGAGCCCCACCGACACAGCCGCCCTGCGGTTTTTGATTGAGTCGCTCAACGACGACGGCTA
TTTGGATGAGCCACTGGAGGACCTTGCGCGTGGCTTGTGCGGCGGCGACTTGGATCAGCTCGATGATTTGGTTCACCACT
TCACGGTCGCCCTGCACTTGTTGCATAGCCTAGAGCCCGTAGGGGTAGGCGCACGCAATTTGGGCGAGTGTTTGCGCTTG
CAGCTCAAAGCCCTGCAGGCGGATGAGGATCTGAGCGATGAGCGCAGCGCCTTGCTGGCGGTGGCCGTGCGGATGTGCTC
CCAGCCCATGGATTTGCTGGCTCGCCGTGACATCAAGCGCTTGGTGCAGCTGTGCGATGCCACCGATGCGCAAGTGCGCG
AAGCCATTGCACTGATCGCGCGCTTGGAACCCAAGCCGGGGCGGCGTTTTGTGGACGTGCAGCGCAACATTGTGGTGCCC
GATGTGCTGGTCGTTCCTGTGAGCAAGCGGCCTGGTGTAGTGAATACCAAGTTTCGGGTACTGCTTAACCCAGAGGTGAT
GCCGCGCTTACGAGTCCATGATATTTATGCCAATGTGCTGCGCAACCACCGTGGCGGCAAAGACACTGCCAGCCACGCTG
CGCTGCAACAGCGCTTGCAAGAAGCCCGCTGGTTCATCAAGAATATCCAGCAGCGCTTTGACACGATTCTGCGGGTGAGC
ACGGCCATCGTCGAGCGGCAAAGTAGTTTTTTTGTGCATGGTGAACTGGCCATGCGCCCCTTGGTGTTGCGTGAAATTGC
GGATGAGCTGGGCTTGCACGAGTCCACCATCAGCCGGGTCACCACGGCCAAGTACATGGCTACGCCCTTTGGTACCTTTG
AGCTGAAATACTTCTTTGGCTCAGGTCTGGGCACCGAGTCGGGGGGTAACGCGTCCAGTACGGCAGTGCGGGCCTTGATC
AAACAATTCATCAGTGCGGAGAGCAATCTCAAACCGCTGTCCGACAACCAAATCTCCGACATGCTCAAAGAGCAGGGCAT
TGAATGTGCCCGCCGTACGGTTGCCAAATACCGCGAAGGGCTCAAAATTGCCCCCGCTTCGCTAAGGAAAGCCCTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-356)

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-530)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.684

100

0.487

Multiple sequence alignment