Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   EXW43_RS08530 Genome accession   NZ_CP036117
Coordinates   1563580..1564182 (-) Length   200 a.a.
NCBI ID   WP_000155584.1    Uniprot ID   J8ATF7
Organism   Bacillus mycoides strain JAS481     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1558580..1569182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW43_RS08510 (EXW43_08720) dnaD 1558865..1559572 (+) 708 WP_000728546.1 DNA replication protein DnaD -
  EXW43_RS08515 (EXW43_08725) nth 1559588..1560235 (+) 648 WP_002030794.1 endonuclease III -
  EXW43_RS08520 (EXW43_08730) - 1560239..1560757 (+) 519 WP_002086586.1 YpoC family protein -
  EXW43_RS08525 (EXW43_08735) - 1560784..1563513 (-) 2730 WP_215576137.1 penicillin-binding protein 1A -
  EXW43_RS08530 (EXW43_08740) recU 1563580..1564182 (-) 603 WP_000155584.1 Holliday junction resolvase RecU Machinery gene
  EXW43_RS08535 (EXW43_08745) - 1564286..1565311 (+) 1026 WP_002126479.1 DUF2515 domain-containing protein -
  EXW43_RS31235 - 1565655..1565801 (-) 147 WP_307838604.1 phage portal protein -
  EXW43_RS08540 (EXW43_08750) - 1565920..1566162 (+) 243 WP_002011865.1 hypothetical protein -
  EXW43_RS08545 (EXW43_08755) - 1566178..1566522 (+) 345 WP_002011864.1 YppE family protein -
  EXW43_RS08550 (EXW43_08760) - 1566548..1566739 (-) 192 WP_002030817.1 YppF family protein -
  EXW43_RS08555 (EXW43_08765) - 1566917..1567507 (+) 591 WP_016126738.1 YppG family protein -
  EXW43_RS08560 (EXW43_08770) - 1567664..1568086 (+) 423 WP_003188340.1 CotD family spore coat protein -
  EXW43_RS08565 (EXW43_08775) - 1568143..1568697 (+) 555 WP_002030808.1 DUF1273 domain-containing protein -
  EXW43_RS08570 (EXW43_08780) gpsB 1568795..1569130 (+) 336 WP_000622426.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23361.62 Da        Isoelectric Point: 9.5601

>NTDB_id=346925 EXW43_RS08530 WP_000155584.1 1563580..1564182(-) (recU) [Bacillus mycoides strain JAS481]
MTIRYPNGKRYDQASQPHKTPIKKHTYSNRGMSLEEELNETNQYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVVAHNGIAFVIIKFTLFDEFYLLDAKHIIAFWNRQ
NTGGRKSITKQEIEEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=346925 EXW43_RS08530 WP_000155584.1 1563580..1564182(-) (recU) [Bacillus mycoides strain JAS481]
ATGACCATTCGTTACCCAAATGGAAAAAGGTACGATCAAGCTTCACAACCTCATAAAACACCAATAAAAAAACATACTTA
CAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACCAATCAATATTACTTAACCCATAATATTGCATGCGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTC
AAACAACCTTCTACAACAGATTACAACGGTGTATACAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACTAAAAATAA
AACCAGTTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAGTCGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAATTTTATTTATTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGTAAATCGATTACAAAACAAGAAATAGAAGAGCATGGGTCTTTATTATCATGCGGTTATCACCCTCG
AATTGATTATATCCGCGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J8ATF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

63

100

0.63

Multiple sequence alignment