Detailed information NTG0346645

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GII86_RS08915
• Genome accession: NZ_CP045816
• Coordinates: 1702331..1703320 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain P5_B2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1697331..1708320

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII86_RS08900 (GII86_08900)	acpP	1697528..1697761 (+)	234	WP_003154310.1	acyl carrier protein	-
GII86_RS08905 (GII86_08905)	rncS	1697901..1698650 (+)	750	WP_003232030.1	ribonuclease III	-
GII86_RS08910 (GII86_08910)	smc	1698751..1702311 (+)	3561	WP_046160400.1	chromosome segregation protein SMC	-
GII86_RS08915 (GII86_08915)	pilA	1702331..1703320 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
GII86_RS08920 (GII86_08920)	sivC	1703356..1703841 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
GII86_RS08925 (GII86_08925)	srpM	1704019..1704351 (+)	333	WP_003238590.1	putative DNA-binding protein	-
GII86_RS08930 (GII86_08930)	ffh	1704365..1705705 (+)	1341	WP_014479771.1	signal recognition particle protein	-
GII86_RS08935 (GII86_08935)	rpsP	1705811..1706083 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
GII86_RS08940 (GII86_08940)	khpA	1706083..1706328 (+)	246	WP_014476792.1	KH domain-containing protein	-
GII86_RS08945 (GII86_08945)	ylqD	1706450..1706836 (+)	387	WP_014476793.1	YlqD family protein	-
GII86_RS08950 (GII86_08950)	rimM	1706841..1707365 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
GII86_RS08955 (GII86_08955)	trmD	1707362..1708093 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=346645 GII86_RS08915 WP_003232026.1 1702331..1703320(+) (pilA) [Bacillus subtilis strain P5_B2]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=346645 GII86_RS08915 WP_003232026.1 1702331..1703320(+) (pilA) [Bacillus subtilis strain P5_B2]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAACTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCCCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAACTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCTGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGTCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAGTTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAGGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGATCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401