Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW38_RS06015 Genome accession   NZ_CP036026
Coordinates   1145110..1148625 (+) Length   1171 a.a.
NCBI ID   WP_078179998.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN54/2     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1140110..1153625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW38_RS05985 (EXW38_06075) - 1140561..1141154 (+) 594 WP_078179996.1 TVP38/TMEM64 family protein -
  EXW38_RS05990 (EXW38_06080) lepB 1141211..1141774 (+) 564 WP_002202818.1 signal peptidase I -
  EXW38_RS05995 (EXW38_06085) - 1142166..1142753 (+) 588 WP_234813916.1 hypothetical protein -
  EXW38_RS06000 (EXW38_06090) - 1142965..1143897 (+) 933 WP_215572531.1 WXG100 family type VII secretion target -
  EXW38_RS06005 (EXW38_06095) - 1143916..1144623 (+) 708 WP_002158921.1 hypothetical protein -
  EXW38_RS06010 (EXW38_06100) - 1144620..1144886 (+) 267 WP_002158920.1 DUF4176 domain-containing protein -
  EXW38_RS06015 (EXW38_06105) addB 1145110..1148625 (+) 3516 WP_078179998.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW38_RS06020 (EXW38_06110) addA 1148622..1152347 (+) 3726 WP_215572532.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW38_RS06025 (EXW38_06115) - 1152371..1152649 (+) 279 WP_078180000.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW38_RS06030 (EXW38_06120) - 1152865..1153572 (+) 708 WP_078180001.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134266.30 Da        Isoelectric Point: 5.2129

>NTDB_id=346019 EXW38_RS06015 WP_078179998.1 1145110..1148625(+) (addB) [Bacillus mycoides strain BPN54/2]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYERIKQVAREEKIEVEKTIPLMEQPRFHSQALAHLEMHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVATEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEARINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMTTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPIDEGMLSDEERNVLNAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=346019 EXW38_RS06015 WP_078179998.1 1145110..1148625(+) (addB) [Bacillus mycoides strain BPN54/2]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GCGAAGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGACT
ATCTGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCGTCTAATGTATATGAAATGTGGCAACAATTAGACACGCATAGCAGTAGTGCAGAGCAAAAGCTACTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATTGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCGGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCTCCTCAAGAGTTAGAAATCGTTAGAGGGCTAATGAGATTAGGAACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAGAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAGGTTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCGCAAGCATTAGCACATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTGACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGAAGACTTGTGGCGACTGAAGACTATCGTTACCGAGATATCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGTATTCGTTCGGCTTTAGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTTCGCTGTGTGAAAACAGAACTTTTATATCCACTAGACGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGCGAGAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAAAGCGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAGCCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTGTCTATGTTTACTGATGTAATGACGACAGGTCTTGAGGCGCTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAATATTGATCATTCTAGATTATCAGATGTTAAAGCGACGTTCATTATTGGCGTAAATGAAGGAG
TGATTCCAGCAGCGCCTATAGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCAACGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACTTTGTCTTACGTCATGCAGCAACTGCAGACGTGGAAGCGTTACGGATTTGAAGGCAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTGTTATCGAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTCGCTCAGCACGGTTTATCGCTAAGAGAGCGCGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAGTTATTCCACGCGGCGCTGAAGAGGATTGCAGACAAGCTATTACGTGAAAATCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCATTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGCTTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGTGGTACTGGATCGCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGCGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGTAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTACGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.063

100

0.492

Multiple sequence alignment