Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   GFD20_RS22065 Genome accession   NZ_CP045768
Coordinates   4823821..4824417 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain CFSAN084950     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4818821..4829417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFD20_RS22045 (GFD20_22045) aroK 4819952..4820470 (-) 519 WP_003095833.1 shikimate kinase AroK -
  GFD20_RS22050 (GFD20_22050) pilQ 4820482..4822626 (-) 2145 WP_014603377.1 type 4a pilus secretin PilQ Machinery gene
  GFD20_RS22055 (GFD20_22055) comL/pilP 4822680..4823204 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  GFD20_RS22060 (GFD20_22060) pilO 4823201..4823824 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  GFD20_RS22065 (GFD20_22065) pilN 4823821..4824417 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  GFD20_RS22070 (GFD20_22070) pilM 4824417..4825481 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  GFD20_RS22075 (GFD20_22075) - 4825666..4828134 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=345908 GFD20_RS22065 WP_003095839.1 4823821..4824417(-) (pilN) [Pseudomonas aeruginosa strain CFSAN084950]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=345908 GFD20_RS22065 WP_003095839.1 4823821..4824417(-) (pilN) [Pseudomonas aeruginosa strain CFSAN084950]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAAGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369