Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EXW60_RS16310 Genome accession   NZ_CP035987
Coordinates   3184411..3187926 (+) Length   1171 a.a.
NCBI ID   WP_215587449.1    Uniprot ID   -
Organism   Bacillus mycoides strain BPN29/1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3179411..3192926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW60_RS16280 (EXW60_16595) cspA 3180003..3180206 (+) 204 WP_002011282.1 RNA chaperone/antiterminator CspA -
  EXW60_RS29610 (EXW60_16600) - 3180233..3180494 (+) 262 Protein_3138 hypothetical protein -
  EXW60_RS16285 (EXW60_16605) - 3180545..3180730 (-) 186 WP_002011281.1 hypothetical protein -
  EXW60_RS16290 (EXW60_16610) - 3181150..3181731 (+) 582 WP_002011279.1 competence protein ComK -
  EXW60_RS16295 (EXW60_16615) - 3181976..3182572 (+) 597 WP_002140800.1 DUF4306 domain-containing protein -
  EXW60_RS16300 (EXW60_16620) - 3182928..3183521 (+) 594 WP_002011278.1 TVP38/TMEM64 family protein -
  EXW60_RS16305 (EXW60_16625) lepB 3183730..3184293 (+) 564 WP_002011277.1 signal peptidase I -
  EXW60_RS16310 (EXW60_16630) addB 3184411..3187926 (+) 3516 WP_215587449.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW60_RS16315 (EXW60_16635) addA 3187923..3191648 (+) 3726 WP_215587450.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW60_RS16320 (EXW60_16640) - 3191672..3191950 (+) 279 WP_215587451.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW60_RS16325 (EXW60_16645) - 3192166..3192873 (+) 708 WP_016119808.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134396.35 Da        Isoelectric Point: 5.1194

>NTDB_id=345579 EXW60_RS16310 WP_215587449.1 3184411..3187926(+) (addB) [Bacillus mycoides strain BPN29/1]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDTHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMRLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKYHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRVRAEENGDFLFATDHEQVWEEVMNLLDTFVEMLGEEK
MSLSMFTEVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=345579 EXW60_RS16310 WP_215587449.1 3184411..3187926(+) (addB) [Bacillus mycoides strain BPN29/1]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GTGAAGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTGCTTCGTAAAATTGTAGAATCTCGTAAAGATGGACT
ATCTGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCTGAGTTTAAACGTTACA
ATGTGACGCCGTCTAACGTATATGAAATGTGGCAACAATTAGACACGCATAGCAGTAGTGCAGAGCAAAAGCTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCTCCTCAAGAGCTAGAAATCGTTAGAGGGCTAATGAGATTAGGAACAAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCTCCAGCATTAGCTCATT
TAGAAACGCATTATGAAGCGCGTCCGAATGAAAAGTATCATGGTGAAGCGAGTGTAACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTAGCTCGTGAAATTAGAAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATATCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGCTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGATGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAGAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGAATTACGTGTTCGTGCAGAAGAAAATGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAATCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTTTCAATGTTTACGGAGGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAACATTGATCATTCTAGATTATCAGATATTAAAGCGACGTTCATTATTGGAGTAAATGAAGGAG
TGATTCCAGCAGCGCCTATGGATGAAGGGATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAAAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACGTTGTCTTATGTCATGCAGCAACTGCAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGTGTGTTATCAAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTTGCCCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGGACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAACACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCGAACCGTCATTTCTATTTAAAGCAAAAACTACAACAAATCATTTTCCGTACGTCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGATTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCTCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGATTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTGCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.147

100

0.492

Multiple sequence alignment