Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   GC231_RS23210 Genome accession   NZ_CP045213
Coordinates   4724677..4725441 (+) Length   254 a.a.
NCBI ID   WP_228400279.1    Uniprot ID   -
Organism   Escherichia coli strain EH41     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4719677..4730441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GC231_RS23190 (GC231_23365) acpT 4720631..4721218 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  GC231_RS23195 (GC231_23370) nikA 4721329..4722903 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  GC231_RS23200 (GC231_23375) nikB 4722903..4723847 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  GC231_RS23205 (GC231_23380) nikC 4723844..4724677 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  GC231_RS23210 (GC231_23385) amiE 4724677..4725441 (+) 765 WP_228400279.1 nickel import ATP-binding protein NikD Regulator
  GC231_RS23215 (GC231_23390) nikE 4725438..4726244 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  GC231_RS23220 (GC231_23395) nikR 4726250..4726651 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  GC231_RS23225 (GC231_23400) - 4726771..4727130 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  GC231_RS23230 (GC231_23405) - 4727127..4727357 (-) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  GC231_RS23235 (GC231_23410) yhhJ 4727461..4728585 (-) 1125 WP_001314210.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26847.44 Da        Isoelectric Point: 6.5992

>NTDB_id=344048 GC231_RS23210 WP_228400279.1 4724677..4725441(+) (amiE) [Escherichia coli strain EH41]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDIETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=344048 GC231_RS23210 WP_228400279.1 4724677..4725441(+) (amiE) [Escherichia coli strain EH41]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATATAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.2

98.425

0.406
  amiE Streptococcus thermophilus LMD-9

41.2

98.425

0.406
  amiE Streptococcus salivarius strain HSISS4

40

98.425

0.394