Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   F4V42_RS12360 Genome accession   NZ_CP044140
Coordinates   2316727..2317491 (-) Length   254 a.a.
NCBI ID   WP_001136210.1    Uniprot ID   Q8X5U1
Organism   Escherichia coli O157 strain AR-0430     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2311727..2322491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F4V42_RS12330 - 2312361..2313719 (-) 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
  F4V42_RS12335 - 2313796..2314077 (-) 282 WP_000084025.1 PTS sugar transporter subunit IIB -
  F4V42_RS12340 - 2314074..2314547 (-) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  F4V42_RS12345 - 2314572..2315318 (-) 747 WP_001301676.1 GntR family transcriptional regulator -
  F4V42_RS12350 nikR 2315517..2315918 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  F4V42_RS12355 nikE 2315924..2316730 (-) 807 WP_000173697.1 nickel import ATP-binding protein NikE -
  F4V42_RS12360 amiE 2316727..2317491 (-) 765 WP_001136210.1 nickel import ATP-binding protein NikD Regulator
  F4V42_RS12365 nikC 2317491..2318324 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  F4V42_RS12370 nikB 2318321..2319265 (-) 945 WP_000947083.1 nickel ABC transporter permease subunit NikB -
  F4V42_RS12375 nikA 2319265..2320839 (-) 1575 WP_000953364.1 nickel ABC transporter substrate-binding protein -
  F4V42_RS12380 acpT 2320950..2321537 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26790.39 Da        Isoelectric Point: 6.5997

>NTDB_id=340916 F4V42_RS12360 WP_001136210.1 2316727..2317491(-) (amiE) [Escherichia coli O157 strain AR-0430]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=340916 F4V42_RS12360 WP_001136210.1 2316727..2317491(-) (amiE) [Escherichia coli O157 strain AR-0430]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTGTCGTTAACCCTGAA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGAATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAGACGTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTTTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCACGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCGCCGGGGATGCTGCTGGTCACCCATGATATGGGCGTGGTGGCGCGTCTGGCGGATGACGTAGCGGTAA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X5U1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398