Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ETK49_RS06255 Genome accession   NZ_CP035405
Coordinates   1215498..1218998 (+) Length   1166 a.a.
NCBI ID   WP_003180427.1    Uniprot ID   A0A415J043
Organism   Bacillus licheniformis strain SRCM103608     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1210498..1223998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK49_RS06230 (ETK49_06230) - 1211157..1212524 (-) 1368 WP_017474228.1 HAMP domain-containing sensor histidine kinase -
  ETK49_RS06235 (ETK49_06235) - 1212521..1213195 (-) 675 WP_009328903.1 response regulator transcription factor -
  ETK49_RS06240 (ETK49_06240) - 1213285..1213725 (-) 441 WP_009328902.1 ferritin-like domain-containing protein -
  ETK49_RS06245 (ETK49_06245) modA 1213929..1214723 (+) 795 WP_003180424.1 molybdate ABC transporter substrate-binding protein -
  ETK49_RS06250 (ETK49_06250) modB 1214732..1215388 (+) 657 WP_003180426.1 molybdate ABC transporter permease subunit -
  ETK49_RS06255 (ETK49_06255) addB 1215498..1218998 (+) 3501 WP_003180427.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ETK49_RS06260 (ETK49_06260) addA 1218985..1222677 (+) 3693 WP_003180429.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ETK49_RS06265 (ETK49_06265) sbcD 1222743..1223924 (+) 1182 WP_003180431.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134468.50 Da        Isoelectric Point: 5.5705

>NTDB_id=340463 ETK49_RS06255 WP_003180427.1 1215498..1218998(+) (addB) [Bacillus licheniformis strain SRCM103608]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLQPESIRDMIEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYADEIRNAAVYVDGFHQFTPQEMNVLEQLMVHAEEITFSLTADKP
FTANSPNDLHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHRHHPELQHLERYFDERPAKPYPGQTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEESGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDDDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDGEEKMLIHEPEQLDDEAQLEFLVNKG
AALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNMISAGLKKDGSLRSDSDVIAEEDFHVLRRHIRRTFQKAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDNVILERLKKEDETDGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=340463 ETK49_RS06255 WP_003180427.1 1215498..1218998(+) (addB) [Bacillus licheniformis strain SRCM103608]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCCGATTTGGGCGGAACCATCCGCGCCCAAGTGTACAGTTTTTCAAGGCTTGCCTGGCGAGTGCTCCAGCATACCGGA
GGCATGAATCGGCCGTTTTTGACGGGGACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCATT
GTCTGCAGCCTGAAAGCATCCGGGACATGATAGAGGGGACGGGCAAATCGGAATACGAAGATGAACGGATCCTTTCCGAT
AAATTGCACGACTTATATATTTTATACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAGGATTATTT
GACGCTTTTGGCCGAGCAGATTCCGTATGCGGATGAGATTAGAAACGCTGCCGTTTACGTGGACGGATTTCACCAATTTA
CGCCACAGGAGATGAACGTGCTGGAACAGCTGATGGTACATGCCGAAGAGATCACGTTTTCGCTGACGGCGGATAAGCCG
TTTACGGCAAACAGCCCGAATGATCTCCATCTGTTCAGGATGACCGGGAAGGCGTACTATGACCTTTACCAAAAGGCGAA
GGAACTCGGCCTTGATCCGTCTGAAGTGCGGCTGGAAGAAACGAAACGGCACCGGCATCATCCGGAGCTTCAGCATCTTG
AACGCTATTTTGATGAACGTCCTGCAAAACCGTATCCCGGACAGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATCCGCCAGGGCCGGTTCCGGCTTAGGGATATTGCTGT
CATCGCCCGCAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGCGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTCATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAACTGGCGGTAT
GAAGCGGTGTTCCGCTGTGTCAAAACGGAACTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCGTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGGT
ACGCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCACCGATCCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACCGTCAGGGAAATGGCCGAAGCCGTTTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTGGCCGAAGAAAGCGGTAGGATTGCAGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATA
TCGTTTGCTTTATTTCAGCAAATGATTGAAACCGGCTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCTCTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGCACATTTCTCATCGGTGTAAACGACGGTATAC
TTCCGGCGAGGCCTGCGGACGACGGCGTGCTTTCAGACGATGACCGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCT
GCGACGGGTCGAGAGCAGCTCTTGGATGAGAATTTTCTGATCTACATGACGCTTTCCAGCCCGTCTGAAAAGCTCTATGT
CTCCTATCCGATCGCAGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTAGTGAAACGGCTGAATGAGCTGTTCCCGG
ACGGCGAAGAGAAGATGCTGATTCATGAACCCGAGCAGCTGGACGACGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
GCCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATTTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAATATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACTTGAACCGGCATGTGAGCCGCGATTTATATGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAAGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGTTTAAAGCTGAAGGAACGTCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCGGCGCTGAAGCTGATTTCAGATCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAATGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTCGCGCCAAAGCTGCAAAAAGAGATTTTGCTCAGTTCGAAC
CGTCATCATTACGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGCGAGCATGCAAAAGCAAG
CGGGTTTGCCCCCGTCGGCATCGAGCTCGGATTCGGGGGGAAAGGTCCGCTTCCTCCAATGCGTTTTACCTTGAAGAACG
GCTGCACAATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTC
GACTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGCCTCGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACACATTCAACCGATTGGCTCGGCATGAAGGCATCCCCTGCAGGCGTGCTTTATTTTCATGTGCACGATC
CGATGATCCAGGCTTCGGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCTTGGGAGACCAGGAAGCGGTCAAGCTGATGGATACCACGCTGGAACAAGGCAGGTCGAACATGATCAGCGCAGGCTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTATTGCAGAAGAGGATTTTCATGTATTGAGGCGCCATATCCGCC
GGACATTTCAGAAGGCCGGCGAGGAGATCACAGACGGTAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCGTACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGGATATTAAAACCCGA
AAAAGACAACGTGATTTTGGAGCGGCTGAAAAAGGAGGATGAGACAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A415J043

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

73.906

99.914

0.738

Multiple sequence alignment