Detailed information NTG0033912

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: VC395_RS02895
• Genome accession: NC_012582
• Coordinates: 586934..587941 (+)
• Length: 335 a.a.
• NCBI ID: WP_000116738.1
• Uniprot ID: A0A0H3AKB4
• Organism: Vibrio cholerae O395
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 581934..592941

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VC395_RS02860 (VC395_0544)	ftsB	581985..582269 (+)	285	WP_001263189.1	cell division protein FtsB	-
VC395_RS02865 (VC395_0545)	ispD	582266..582964 (+)	699	WP_001061948.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
VC395_RS02870 (VC395_0546)	ispF	582961..583437 (+)	477	WP_000619209.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
VC395_RS02875 (VC395_0547)	truD	583467..584552 (+)	1086	WP_000129752.1	tRNA pseudouridine(13) synthase TruD	-
VC395_RS02880 (VC395_0548)	surE	584554..585306 (+)	753	WP_000698379.1	5'/3'-nucleotidase SurE	-
VC395_RS02885 (VC395_0549)	-	585299..585925 (+)	627	WP_000002982.1	protein-L-isoaspartate(D-aspartate) O-methyltransferase	-
VC395_RS02890 (VC395_0550)	nlpD	585925..586860 (+)	936	WP_000177568.1	murein hydrolase activator NlpD	-
VC395_RS02895 (VC395_0551)	rpoS	586934..587941 (+)	1008	WP_000116738.1	RNA polymerase sigma factor RpoS	Regulator
VC395_RS02900 (VC395_0552)	mutS	588034..590622 (-)	2589	WP_001894770.1	DNA mismatch repair protein MutS	-
VC395_RS02905 (VC395_0554)	cysM	590890..591777 (-)	888	WP_001279365.1	cysteine synthase CysM	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 38507.37 Da
• Isoelectric Point: 4.3648

>NTDB_id=33912 VC395_RS02895 WP_000116738.1 586934..587941(+) (rpoS) [Vibrio cholerae O395]
MSVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLY
ARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALM
NQTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVK
QGLNMEALFSVEYDN

Nucleotide

• Length: 1008 bp

>NTDB_id=33912 VC395_RS02895 WP_000116738.1 586934..587941(+) (rpoS) [Vibrio cholerae O395]
ATGAGTGTCAGCAATACCGTAACCAAAGTAGAAGAGTTCGATTTTGAAGATGAAGCACTGGAAGTGCTAGAAACTGATGC
CGAGCTCACCAGTGATGAAGAATTAGTTGCTGTTGAAGGGGCAAGTGAAGACGTTCGTGAAGAGTTTGATGCTTCTGCGA
AAAGTCTTGATGCGACCCAGATGTATCTCAGCGAAATTGGTTTTTCACCGCTCCTTACTGCCGAAGAAGAAGTGCTTTAT
GCTCGTCGTGCCTTACGTGGTGATGAAGCCGCACGTAAACGCATGATTGAAAGTAACTTGCGTCTGGTGGTAAAAATTTC
ACGCCGTTACAGCAACCGAGGATTAGCACTGCTCGATCTGATTGAAGAAGGTAATCTTGGTCTGATCCGTGCGGTTGAGA
AATTCGATCCAGAACGCGGTTTCCGCTTCTCTACCTACGCAACATGGTGGATCCGTCAAACCATTGAACGTGCGCTGATG
AACCAAACACGCACCATTCGTCTACCGATTCATGTCGTCAAAGAGCTGAACATTTATTTGCGTACTGCTCGCGAATTATC
ACAGCGCCTTGACCACGAACCTACACCAGAAGAAATTGCTCTTGAGTTAGACCGACCTGTCGATGATGTCACTAAGATGC
TGCGTCTTAACGAACGGATCAGCTCAGTGGATACGCCAATTGGTGGGGATGGAGATAAGGCACTGCTGGATATTTTGCCA
GACTCTCACAATGCCGATCCTGAGTTTTCAACTCAAGATGATGACATTCGTGAATCGCTGCTCAACTGGTTGGATGAACT
TAATCCAAAGCAAAAAGAAGTGCTTGCGCGTCGCTTTGGGCTTCTTGGCTATGAACCATCGACCTTGGAAGAAGTGGGTC
GTGAGATCAATCTCACTCGTGAGCGTGTTCGCCAAATCCAAGTGGAAGGTCTACGTCGTCTGCGTGAGATTTTGGTGAAA
CAAGGTTTGAATATGGAAGCGCTGTTTAGCGTCGAATACGACAACTAA

Domains

Predicted by InterproScan.

• Sigma-70, region 4 (Sigma70_r4) (263-316)

• Sigma-70 region 3 (Sigma70_r3) (175-249)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (57-90)

• Sigma-70 region 2 (Sigma70_r2) (95-164)

3D structure

• AlphaFold DB: A0A0H3AKB4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	99.701	100	0.997