Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   VCM66_RS19960 Genome accession   NC_012578
Coordinates   2830907..2831767 (+) Length   286 a.a.
NCBI ID   WP_001884047.1    Uniprot ID   Q9KNL3
Organism   Vibrio cholerae M66-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825907..2836767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCM66_RS13010 (VCM66_2635) greB 2826435..2826920 (-) 486 WP_000850496.1 transcription elongation factor GreB -
  VCM66_RS13015 (VCM66_2636) - 2827165..2829486 (+) 2322 WP_000030087.1 Tex family protein -
  VCM66_RS13020 (VCM66_2637) - 2829578..2830048 (-) 471 WP_000961392.1 hypothetical protein -
  VCM66_RS13025 (VCM66_2638) bioH 2830210..2830971 (-) 762 WP_001240449.1 pimeloyl-ACP methyl ester esterase BioH -
  VCM66_RS19960 (VCM66_2639) comF 2830907..2831767 (+) 861 WP_001884047.1 ComF family protein Machinery gene
  VCM66_RS13035 (VCM66_2640) nfuA 2831855..2832442 (+) 588 WP_000070178.1 Fe-S biogenesis protein NfuA -
  VCM66_RS13040 (VCM66_2641) nudE 2832581..2833129 (+) 549 WP_000095812.1 ADP compounds hydrolase NudE -
  VCM66_RS13045 (VCM66_2642) cysQ 2833152..2833979 (+) 828 WP_000106799.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  VCM66_RS13050 (VCM66_2643) - 2834031..2834789 (-) 759 WP_000817146.1 type II secretion system protein N -
  VCM66_RS13055 (VCM66_2644) gspM 2834791..2835288 (-) 498 WP_000661339.1 type II secretion system protein GspM -
  VCM66_RS13060 (VCM66_2645) gspL 2835298..2836509 (-) 1212 WP_001288747.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32545.47 Da        Isoelectric Point: 8.0588

>NTDB_id=33848 VCM66_RS19960 WP_001884047.1 2830907..2831767(+) (comF) [Vibrio cholerae M66-2]
MPQPCTNTRSCPCPLTCQYNAMVTVSPSISIFIHSANWQDEAHYPDSWLAMLTHWLRKTTAPLLTPECHLCRLALDTNSP
FGVCSACQAWLEHGYRCARCGLPTLTPVDQCGQCLGQPPPWRKLMCVGDYRFPLSDAVHQLKYQRQFWQAPRLAKLLATQ
INEPAPLLCSVPLHWRRRWQRGFNQSDLLARELANMLNVEYDHQLFARRRATPHQQGLSKAQRIHNLRDAFVLNHPPNQP
HVAIVDDVVTTGSTIRHLCDLLLDVGVQSIDIYCICRTPEPKDSHG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=33848 VCM66_RS19960 WP_001884047.1 2830907..2831767(+) (comF) [Vibrio cholerae M66-2]
ATGCCCCAGCCGTGTACCAATACCAGATCTTGCCCTTGTCCACTGACTTGCCAATACAACGCCATGGTCACAGTTTCTCC
GTCTATTTCCATCTTCATCCACTCTGCCAATTGGCAAGATGAAGCACATTACCCCGATTCTTGGCTCGCTATGTTAACCC
ATTGGCTCAGAAAAACTACCGCACCACTGCTGACTCCGGAATGTCATCTCTGTCGACTGGCGCTAGATACAAATTCGCCG
TTTGGCGTCTGCTCTGCCTGCCAAGCTTGGCTTGAGCACGGTTATCGCTGCGCACGCTGCGGTTTACCGACTCTTACCCC
AGTTGATCAGTGCGGGCAGTGTTTGGGTCAGCCTCCGCCGTGGCGAAAACTGATGTGTGTCGGCGATTACCGCTTTCCAC
TTAGTGATGCGGTGCATCAACTCAAATACCAACGCCAGTTTTGGCAAGCGCCGCGCTTGGCTAAGCTACTCGCCACCCAG
ATTAACGAGCCAGCCCCGTTGCTGTGCAGCGTGCCACTGCATTGGCGGCGACGCTGGCAACGCGGCTTTAATCAAAGCGA
TCTGCTGGCGCGCGAGTTAGCCAATATGCTGAACGTTGAATATGACCACCAACTCTTTGCACGCCGCCGAGCGACACCGC
ATCAACAAGGACTCAGCAAGGCGCAGCGAATCCACAACCTACGCGATGCGTTTGTGCTGAATCATCCGCCAAACCAACCA
CATGTTGCGATTGTGGACGATGTGGTGACCACAGGCAGTACGATCCGACATTTATGCGATTTACTGCTTGATGTCGGTGT
GCAAAGCATTGATATTTACTGCATATGCCGCACTCCCGAGCCGAAAGATAGCCACGGTTGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(189-281)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KNL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Vibrio cholerae strain A1552

100

100

1
  comF Vibrio campbellii strain DS40M4

47.059

83.217

0.392

Multiple sequence alignment