Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilP   Type   Machinery gene
Locus tag   VCM66_RS12625 Genome accession   NC_012578
Coordinates   2740034..2740552 (-) Length   172 a.a.
NCBI ID   WP_000792032.1    Uniprot ID   -
Organism   Vibrio cholerae M66-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735034..2745552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCM66_RS12610 (VCM66_2548) aroB 2736469..2737554 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  VCM66_RS12615 (VCM66_2549) aroK 2737582..2738106 (-) 525 WP_000818616.1 shikimate kinase AroK -
  VCM66_RS12620 (VCM66_2550) pilQ 2738293..2740008 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  VCM66_RS12625 (VCM66_2551) pilP 2740034..2740552 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  VCM66_RS12630 (VCM66_2552) pilO 2740542..2741132 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCM66_RS12635 (VCM66_2553) pilN 2741125..2741709 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  VCM66_RS12640 (VCM66_2554) pilM 2741699..2742715 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  VCM66_RS12645 (VCM66_2555) - 2742858..2745335 (+) 2478 WP_000668733.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 19084.25 Da        Isoelectric Point: 10.0857

>NTDB_id=33840 VCM66_RS12625 WP_000792032.1 2740034..2740552(-) (pilP) [Vibrio cholerae M66-2]
MKNKPLGLWLMSLLVLVGCKANQDDLTSYVAQVEREARKEVTKLKPILEFEATPYQQHKGREPFVLPKEALVQNQPLAKA
DCWQPVPRAKNGPLERYPLHQLRLKGVMSSGGSISALVQTPAGSVVKVKAGQYVGINNGKVTRVDDNYLLINETLPDGLG
CWNQRNVKLALK

Nucleotide


Download         Length: 519 bp        

>NTDB_id=33840 VCM66_RS12625 WP_000792032.1 2740034..2740552(-) (pilP) [Vibrio cholerae M66-2]
ATGAAAAATAAGCCACTTGGATTATGGCTGATGAGTTTGCTGGTGTTGGTCGGGTGTAAGGCGAACCAAGATGATTTGAC
CTCATACGTCGCGCAAGTTGAGCGTGAAGCGCGCAAAGAAGTAACTAAGCTCAAACCCATTTTAGAGTTTGAAGCCACGC
CCTATCAGCAGCACAAGGGGCGTGAGCCGTTTGTCTTACCGAAAGAAGCGTTAGTGCAAAACCAACCGCTGGCCAAAGCC
GACTGTTGGCAACCCGTGCCACGTGCGAAAAATGGGCCGTTGGAGCGTTATCCATTGCACCAATTGCGCTTAAAAGGGGT
GATGAGCAGCGGGGGCAGTATCTCTGCCTTAGTGCAAACGCCGGCAGGGAGTGTGGTCAAGGTTAAGGCGGGTCAATATG
TCGGAATCAATAATGGCAAAGTCACCCGAGTCGACGACAACTATTTGTTAATTAATGAAACTTTGCCTGATGGCTTAGGG
TGTTGGAATCAGCGTAACGTCAAGCTGGCTTTAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilP (PilP)

(25-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilP Vibrio cholerae strain A1552

100

100

1
  pilP Vibrio campbellii strain DS40M4

56.395

100

0.564

Multiple sequence alignment