Detailed information NTG0337998

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: FTN78_RS00500
• Genome accession: NZ_CP043523
• Coordinates: 46573..48912 (+)
• Length: 779 a.a.
• NCBI ID: WP_003131470.1
• Uniprot ID: Q9CEE5
• Organism: Lactococcus lactis subsp. lactis bv. diacetylactis strain SD96
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 41573..53912

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FTN78_RS00480 (FTN78_chr0047)	-	42597..43310 (+)	714	WP_003131466.1	glucosamine-6-phosphate deaminase	-
FTN78_RS00485 (FTN78_chr0048)	rny	43541..45136 (+)	1596	WP_003131467.1	ribonuclease Y	-
FTN78_RS00490 (FTN78_chr0049)	gmk	45284..45901 (+)	618	WP_003131468.1	guanylate kinase	-
FTN78_RS00495 (FTN78_chr0050)	rpoZ	46081..46434 (+)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
FTN78_RS00500 (FTN78_chr0051)	priA	46573..48912 (+)	2340	WP_003131470.1	primosomal protein N'	Machinery gene
FTN78_RS00505 (FTN78_chr0052)	-	48971..49345 (+)	375	WP_010906188.1	nuclear transport factor 2 family protein	-
FTN78_RS00510 (FTN78_chr0053)	-	49358..49879 (+)	522	WP_003131472.1	NUDIX hydrolase	-
FTN78_RS00515 (FTN78_chr0054)	-	49845..50309 (+)	465	WP_003131474.1	VOC family protein	-
FTN78_RS00520 (FTN78_chr0055)	fmt	50306..51265 (+)	960	WP_010906187.1	methionyl-tRNA formyltransferase	-
FTN78_RS00525 (FTN78_chr0056)	-	51440..51682 (+)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
FTN78_RS00530 (FTN78_chr0057)	amaP	51710..52270 (+)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
FTN78_RS00535 (FTN78_chr0058)	-	52281..52469 (+)	189	WP_012898445.1	DUF2273 domain-containing protein	-
FTN78_RS00540 (FTN78_chr0059)	-	52490..52996 (+)	507	WP_003131479.1	Asp23/Gls24 family envelope stress response protein	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88929.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=337998 FTN78_RS00500 WP_003131470.1 46573..48912(+) (priA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain SD96]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENK
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVRSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=337998 FTN78_RS00500 WP_003131470.1 46573..48912(+) (priA) [Lactococcus lactis subsp. lactis bv. diacetylactis strain SD96]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTCCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAAA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCTGTCAGATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAAGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCTGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Type III restriction enzyme, res subunit (ResIII) (247-405)

3D structure

• AlphaFold DB: Q9CEE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.637	100	0.492