Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EQI27_RS14165 Genome accession   NZ_CP035163
Coordinates   2703355..2705715 (-) Length   786 a.a.
NCBI ID   WP_015714440.1    Uniprot ID   A0A8E0VQW3
Organism   Bacillus subtilis strain SRCM103923     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2698355..2710715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI27_RS14145 (EQI27_14145) yrvJ 2698429..2699985 (+) 1557 WP_029727135.1 SH3 domain-containing protein -
  EQI27_RS14150 (EQI27_14150) dtd 2700012..2700452 (-) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  EQI27_RS14155 (EQI27_14155) relA 2700465..2702669 (-) 2205 WP_003229747.1 GTP diphosphokinase -
  EQI27_RS14160 (EQI27_14160) aptA 2702837..2703349 (-) 513 WP_003229745.1 adenine phosphoribosyltransferase -
  EQI27_RS14165 (EQI27_14165) recJ 2703355..2705715 (-) 2361 WP_015714440.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EQI27_RS14170 (EQI27_14170) yrvD 2705782..2706105 (-) 324 WP_003229740.1 lipopolysaccharide assembly LapA domain-containing protein -
  EQI27_RS14175 (EQI27_14175) yrvC 2706181..2706678 (-) 498 WP_003229739.1 cation:proton antiporter regulatory subunit -
  EQI27_RS14180 (EQI27_14180) secDF 2706836..2709049 (-) 2214 WP_015714442.1 protein translocase subunit SecDF -
  EQI27_RS14185 (EQI27_14185) comN 2709088..2709384 (-) 297 WP_003229735.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88019.66 Da        Isoelectric Point: 5.1797

>NTDB_id=335133 EQI27_RS14165 WP_015714440.1 2703355..2705715(-) (recJ) [Bacillus subtilis strain SRCM103923]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKDRGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCSVEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELQEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSALPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSKDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=335133 EQI27_RS14165 WP_015714440.1 2703355..2705715(-) (recJ) [Bacillus subtilis strain SRCM103923]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTGATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGATCGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTGGATGTCATCATTACCGATCATCATGAACCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACTTACCCGTTTAAAGAGCTTGCCGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTTTATTGGGTGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACAGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTCGGAAGAATCGAACAGGCGGATC
CAGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCCGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGCTTCTACCGACCGGCCA
TTGTTCTTGGCATTGATGAAGAAAAGGGAATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCTGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAACGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCAGCGTCGAAGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTTAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTACTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTCAAGAAGGCATCGTACCGGGTT
CAAGAATTTCCATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGCCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACATTACTTCAAACTGAAGACCTTCGCCGAGAAGTGCACGTCATCA
GTTCTAAAGATCAAGCAAAGGCGTTTGACCTCGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCCCGTTTGCTGGAAGGCAAGGCGCCTGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGAAAAAACACGGTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHHA1 domain (DHHA1)

(315-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

99.618

100

0.996

Multiple sequence alignment