Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   BSN5_RS05055 Genome accession   NC_014976
Coordinates   929458..931815 (-) Length   785 a.a.
NCBI ID   WP_015714480.1    Uniprot ID   -
Organism   Bacillus subtilis BSn5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 924458..936815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSN5_RS05030 (BSn5_05090) etfB 924900..925673 (-) 774 WP_014480500.1 electron transfer flavoprotein subunit beta -
  BSN5_RS05035 (BSn5_05095) fadB 925688..926464 (-) 777 WP_015714478.1 enoyl-CoA hydratase -
  BSN5_RS05040 (BSn5_05100) fadR 926479..927063 (-) 585 WP_003229547.1 fatty acid metabolism transcriptional regulator FadR -
  BSN5_RS05045 (BSn5_05105) lcfA 927168..928850 (-) 1683 WP_015714479.1 long-chain-fatty-acid--CoA ligase LcfA -
  BSN5_RS05050 (BSn5_05110) yshE 929039..929443 (-) 405 WP_003237674.1 DUF350 domain-containing protein -
  BSN5_RS05055 (BSn5_05115) mutS/mutS2 929458..931815 (-) 2358 WP_015714480.1 endonuclease MutS2 Machinery gene
  BSN5_RS05060 (BSn5_05120) polX 931836..933548 (-) 1713 WP_015714481.1 DNA polymerase/3'-5' exonuclease PolX -
  BSN5_RS05065 (BSn5_05125) yshB 933623..934156 (-) 534 WP_003229537.1 CvpA family protein -
  BSN5_RS05070 (BSn5_05130) zapA 934163..934420 (-) 258 WP_003229534.1 cell division protein ZapA -
  BSN5_RS05075 (BSn5_05135) rnhC 934554..935492 (+) 939 WP_015251512.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87384.75 Da        Isoelectric Point: 6.1361

>NTDB_id=33420 BSN5_RS05055 WP_015714480.1 929458..931815(-) (mutS/mutS2) [Bacillus subtilis BSn5]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEYTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPDQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEEAEQQAAEKVKAAMKEAEDIIHELRSIKEEHKSFKDHELINAKKRLEGAMPAFEKSKKPEKPKTQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEPKKEKIITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=33420 BSN5_RS05055 WP_015714480.1 929458..931815(-) (mutS/mutS2) [Bacillus subtilis BSn5]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCTTCTGCTTCTATAGACGAAATCAAAAAACAGCTGGATGAAGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGTGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
CCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATTGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACATTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCATCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACACTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAGCAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGTATACAGAGGAGCTATT
TCTAGATTTGCAAGTGCTGCAGACGCTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATCCGTTTGAAAAAAGCCCGCCATCCATTGCTTCCGCCTGATCAGGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACCATTGTCATCACAGGGCCAAACACCGGGGGGAAGACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAGTCAGGTCTTCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGTGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCATATGGTGAATATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGCCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATCTTGGATGACGTGCATCGCACCAATGCACGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCCAGCGTTGAATTTGACATCGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGTCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTTCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAACAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAGACAGAATCAATCAGAAAAGAAGCGGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGAGGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATGAAAGAAGC
CGAGGACATTATTCATGAATTGCGCTCCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATTAACGCGAAGA
AACGGTTAGAAGGCGCTATGCCTGCTTTTGAAAAGTCCAAGAAACCGGAAAAGCCGAAAACGCAAAAACGCGATTTTAAG
CCTGGCGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACATTGCTCGAAAAAACAGGCGGCAATGAATGGAATGT
TCAAATCGGTATTTTAAAGATGAAAGTAAAAGAAAAAGATCTGGAGTTTATCAAATCAGCTCCGGAGCCAAAAAAAGAAA
AAATCATTACAGCGGTCAAGGGAAAGGACTATCACGTATCGCTTGAACTTGATCTCCGCGGCGAACGCTATGAAAATGCC
CTAAGCCGGGTTGAAAAATACTTGGATGATGCGGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCACCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.745

100

0.997

Multiple sequence alignment