Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EKQ63_RS07370 Genome accession   NZ_CP034686
Coordinates   1059605..1063120 (+) Length   1171 a.a.
NCBI ID   WP_143880923.1    Uniprot ID   A0A516QNC9
Organism   Bacillus sp. BD59S     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1054605..1068120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKQ63_RS07330 (EKQ63_07310) cspA 1055107..1055310 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  EKQ63_RS07335 (EKQ63_07315) - 1055501..1055713 (-) 213 WP_000412670.1 hypothetical protein -
  EKQ63_RS07340 (EKQ63_07320) - 1056033..1056218 (-) 186 WP_087951638.1 hypothetical protein -
  EKQ63_RS07345 (EKQ63_07325) - 1056503..1057084 (+) 582 WP_044792696.1 competence protein ComK -
  EKQ63_RS07350 (EKQ63_07330) - 1057324..1057920 (+) 597 WP_087951639.1 DUF4306 domain-containing protein -
  EKQ63_RS07360 (EKQ63_07340) - 1058275..1058868 (+) 594 WP_048553998.1 TVP38/TMEM64 family protein -
  EKQ63_RS07365 (EKQ63_07345) lepB 1058925..1059488 (+) 564 WP_000751900.1 signal peptidase I -
  EKQ63_RS07370 (EKQ63_07350) addB 1059605..1063120 (+) 3516 WP_143880923.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EKQ63_RS07375 (EKQ63_07355) addA 1063117..1066842 (+) 3726 WP_143880924.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EKQ63_RS07380 (EKQ63_07360) - 1066855..1067142 (+) 288 WP_087951643.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EKQ63_RS07385 (EKQ63_07365) gerPF 1067247..1067462 (-) 216 WP_001141566.1 spore germination protein GerPF -
  EKQ63_RS07390 (EKQ63_07370) - 1067505..1067891 (-) 387 WP_087951644.1 spore germination protein GerPE -
  EKQ63_RS07395 (EKQ63_07375) gerPD 1067907..1068101 (-) 195 WP_001102341.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134301.30 Da        Isoelectric Point: 5.1771

>NTDB_id=332478 EKQ63_RS07370 WP_143880923.1 1059605..1063120(+) (addB) [Bacillus sp. BD59S]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEVGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQSLAHLEAHYEARPNEKFHGEASVTIHTAANL
RAEVEGVAREIRRLVADENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=332478 EKQ63_RS07370 WP_143880923.1 1059605..1063120(+) (addB) [Bacillus sp. BD59S]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGTGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTCGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTGCTAGTAGAAAAGCTTCCGCAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TCTCACCGCAGGAGCTAGAAATTGTAAGGCAGCTTATGATTTGCGGAGCGAGAGTGACAATCACGTTAACGCTAGATGAA
AAAACGTTAGCACAACCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCGCAATCATTAGCGCATT
TAGAAGCGCACTACGAAGCACGACCAAATGAAAAATTTCACGGAGAAGCAAGTGTAACGATTCATACAGCCGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCACGTGAAATTCGTAGACTTGTGGCGGACGAAAACTACCGTTACCGAGATATTGCGGT
TCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTGGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTTCTGAAGATCCATGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGGACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTACGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACGGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAGAAGTTAGAGGAATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGTG
TGATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAAAAATTGTA
TATTTCGTGTCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCATCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGATGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACATTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGCAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGGAGCGTCTCTCGTATGGAG
TTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATTGGGGAATTATTCCATGCAGCACTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAGCA
CGCAAAATCGAGTGGTTTCGTACCAGTTGATTTAGAAGTACCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGTCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCGCATACGTGGATGAAAAAAGGCGGCACGGCATCACCAGCTGGTG
TATTGTATTTCCACATTCATAACCCAATTGTGGAGGTAAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGGAAAGATATTACAGAAGGCGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCGGTTTGCCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A516QNC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.233

100

0.494

Multiple sequence alignment