Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   FVE22_RS21095 Genome accession   NZ_CP042844
Coordinates   4323861..4324625 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain JME66     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4318861..4329625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVE22_RS21075 acpT 4319815..4320402 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  FVE22_RS21080 nikA 4320513..4322087 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  FVE22_RS21085 nikB 4322087..4323031 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  FVE22_RS21090 nikC 4323028..4323861 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  FVE22_RS21095 amiE 4323861..4324625 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  FVE22_RS21100 nikE 4324622..4325428 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  FVE22_RS21105 nikR 4325434..4325835 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=332113 FVE22_RS21095 WP_001136229.1 4323861..4324625(+) (amiE) [Escherichia coli strain JME66]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=332113 FVE22_RS21095 WP_001136229.1 4323861..4324625(+) (amiE) [Escherichia coli strain JME66]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment