Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NX81_RS17470 Genome accession   NZ_CP034649
Coordinates   3743219..3744952 (-) Length   577 a.a.
NCBI ID   WP_039432925.1    Uniprot ID   -
Organism   Xanthomonas vasicola strain NCPPB 1060     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3738219..3749952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX81_RS17455 (NX81_017455) - 3739274..3740887 (+) 1614 WP_017117852.1 HAMP domain-containing sensor histidine kinase -
  NX81_RS17460 (NX81_017460) pilR 3741152..3742606 (+) 1455 WP_039446302.1 sigma-54 dependent transcriptional regulator Regulator
  NX81_RS17470 (NX81_017470) pilB 3743219..3744952 (-) 1734 WP_039432925.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NX81_RS17475 (NX81_017475) pilA/pilAI 3745081..3745488 (-) 408 WP_039433041.1 pilin Machinery gene
  NX81_RS17480 (NX81_017480) pilC 3745840..3747093 (+) 1254 WP_039433039.1 type II secretion system F family protein Machinery gene
  NX81_RS17485 (NX81_017485) - 3747100..3747963 (+) 864 WP_039446301.1 A24 family peptidase -
  NX81_RS17490 (NX81_017490) coaE 3747977..3748618 (+) 642 WP_039432920.1 dephospho-CoA kinase -
  NX81_RS17495 (NX81_017495) - 3748937..3749920 (-) 984 WP_053013007.1 IS5 family transposase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62687.88 Da        Isoelectric Point: 5.4566

>NTDB_id=332113 NX81_RS17470 WP_039432925.1 3743219..3744952(-) (pilB) [Xanthomonas vasicola strain NCPPB 1060]
MSVAGAANLVGITGIARRLVQDGALEELAARAAMDQAAVAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDSS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDDEMGDLDVSTGDEDMGAGGDSGVDARGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPEQIANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=332113 NX81_RS17470 WP_039432925.1 3743219..3744952(-) (pilB) [Xanthomonas vasicola strain NCPPB 1060]
ATGAGTGTTGCTGGAGCAGCCAATTTAGTTGGCATCACAGGGATTGCTCGCCGACTAGTTCAGGATGGCGCACTGGAAGA
ATTGGCGGCACGTGCCGCCATGGACCAAGCGGCCGTGGCCAAGGTCCCTTTGCCACAATGGTTTGCCGAAAAAAAATTAG
TGTCGGCGGCGCAGCTAGCGGCCGCTAACGCAGTCGAGTTCGGCATGCCGTTGATGGACGTATCAGTGTTCGATTCTAGC
CAGAACGCCGTCAAGCTGGTCAGCGAAGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGTGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACTCGCGCGCTGGACGACATCAAGTTTCATACGAACCTGGTGGTCGAGGCGA
TCCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGT
AATGACGACGATGAGATGGGGGACTTGGACGTCTCCACTGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAGGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGTTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCAAAGGCGCCG
GTGAAGCTCAATCAACGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCTAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGCGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCTGACCAGCAGAAG
CTGTTCCTGGATGCGATCCATAAGCCGTATGGCATGGTGCTGGTGACCGGGCCTACGGGCTCGGGTAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGGAACATTTCCACGGCAGAGGATCCGGTCGAAATTCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTTGCCGCTGCGCTGCGTTCGTTCCTGCGTCAGGACCCG
GACATCATTATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCAGCGCAGACGGGCCACATGGT
GCTGTCGACGCTGCACACTAACGATGCGCCGCAGACCATTGCGCGTCTGATGAATATGGGGATCGCGCCTTACAACATCA
CCTCGTCGGTAACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGTCGACGTTGCCG
GACAACGCATTGCTGGCGGAAGGTTTCACGCCCGAGCAAATTGCGAACATGGAGCTCTACGAGGCTGTGGGTTGCGACGA
GTGCACTGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGAAAATCGGTATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGGGTCACCAGCCTGGCCGAGATCAATCGTGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.44

0.551
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio cholerae strain A1552

49.365

95.494

0.471
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

45.115

97.574

0.44

Multiple sequence alignment