Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EKH72_RS01755 Genome accession   NZ_CP034565
Coordinates   334539..335555 (+) Length   338 a.a.
NCBI ID   WP_129829803.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain D3112     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329539..340555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKH72_RS01740 - 329963..330691 (-) 729 WP_015297397.1 redoxin family protein -
  EKH72_RS01745 oxyR 330862..331770 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  EKH72_RS01750 - 331826..334396 (-) 2571 WP_129829802.1 PBP1A family penicillin-binding protein -
  EKH72_RS01755 pilM 334539..335555 (+) 1017 WP_129829803.1 type IV pilus assembly protein PilM Machinery gene
  EKH72_RS01760 pilN 335539..336117 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  EKH72_RS01765 pilO 336110..336703 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  EKH72_RS01770 pilP 336693..337208 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  EKH72_RS01775 pilQ 337240..338988 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  EKH72_RS01780 aroK 339177..339695 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.30 Da        Isoelectric Point: 7.0223

>NTDB_id=331914 EKH72_RS01755 WP_129829803.1 334539..335555(+) (pilM) [Vibrio parahaemolyticus strain D3112]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEKFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=331914 EKH72_RS01755 WP_129829803.1 334539..335555(+) (pilM) [Vibrio parahaemolyticus strain D3112]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCAAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAAAAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGCTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment