Detailed information NTG0329637

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BVMH_RS12995
• Genome accession: NZ_CP034176
• Coordinates: 2645345..2645959 (-)
• Length: 204 a.a.
• NCBI ID: WP_043021637.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain MH25
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2640345..2650959

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVMH_RS12965 (BVMH_12965)	rpsT	2640648..2640914 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BVMH_RS12970 (BVMH_12970)	holA	2640931..2641972 (-)	1042	Protein_2503	DNA polymerase III subunit delta	-
BVMH_RS12975 (BVMH_12975)	-	2642012..2642163 (-)	152	Protein_2504	hypothetical protein	-
BVMH_RS12980 (BVMH_12980)	-	2642204..2642338 (+)	135	WP_003152870.1	YqzM family protein	-
BVMH_RS12985 (BVMH_12985)	comEC	2642357..2644708 (-)	2352	WP_094031847.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BVMH_RS12990 (BVMH_12990)	-	2644709..2645278 (-)	570	WP_094031848.1	ComE operon protein 2	-
BVMH_RS12995 (BVMH_12995)	comEA	2645345..2645959 (-)	615	WP_043021637.1	helix-hairpin-helix domain-containing protein	Machinery gene
BVMH_RS13000 (BVMH_13000)	comER	2646018..2646839 (+)	822	WP_012118027.1	late competence protein ComER	-
BVMH_RS13005 (BVMH_13005)	-	2646908..2647645 (-)	738	WP_076424998.1	class I SAM-dependent methyltransferase	-
BVMH_RS13010 (BVMH_13010)	rsfS	2647642..2647998 (-)	357	WP_007408260.1	ribosome silencing factor	-
BVMH_RS13015 (BVMH_13015)	yqeK	2648016..2648576 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BVMH_RS13020 (BVMH_13020)	-	2648566..2649135 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BVMH_RS13025 (BVMH_13025)	yhbY	2649146..2649436 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
BVMH_RS13030 (BVMH_13030)	aroE	2649430..2650272 (-)	843	WP_094031849.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22165.31 Da
• Isoelectric Point: 6.4888

>NTDB_id=329637 BVMH_RS12995 WP_043021637.1 2645345..2645959(-) (comEA) [Bacillus velezensis strain MH25]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=329637 BVMH_RS12995 WP_043021637.1 2645345..2645959(-) (comEA) [Bacillus velezensis strain MH25]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363