Detailed information NTG0326568

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: FNE80_RS01240
• Genome accession: NZ_CP041437
• Coordinates: 272865..273629 (-)
• Length: 254 a.a.
• NCBI ID: WP_001136236.1
• Uniprot ID: A7ZT17
• Organism: Escherichia coli strain STEC005
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 267865..278629

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNE80_RS01200 (FNE80_01245)	yhhJ	268460..269584 (+)	1125	WP_001314210.1	ABC transporter permease	-
FNE80_RS01205 (FNE80_01250)	-	269688..269918 (+)	231	WP_042014162.1	type II toxin-antitoxin system HicA family toxin	-
FNE80_RS01210 (FNE80_01255)	-	269915..270052 (+)	138	Protein_243	type II toxin-antitoxin system HicB family antitoxin	-
FNE80_RS01220 (FNE80_01260)	-	270119..271281 (+)	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
FNE80_RS01225 (FNE80_01265)	-	271311..271535 (+)	225	Protein_245	type II toxin-antitoxin system HicB family antitoxin	-
FNE80_RS01230 (FNE80_01270)	nikR	271655..272056 (-)	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
FNE80_RS01235 (FNE80_01275)	nikE	272062..272868 (-)	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
FNE80_RS01240 (FNE80_01280)	amiE	272865..273629 (-)	765	WP_001136236.1	nickel import ATP-binding protein NikD	Regulator
FNE80_RS01245 (FNE80_01285)	nikC	273629..274462 (-)	834	WP_001008968.1	nickel ABC transporter permease subunit NikC	-
FNE80_RS01250 (FNE80_01290)	nikB	274459..275403 (-)	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
FNE80_RS01255 (FNE80_01295)	nikA	275403..276977 (-)	1575	WP_000953353.1	nickel ABC transporter substrate-binding protein	-
FNE80_RS01260 (FNE80_01300)	acpT	277088..277675 (-)	588	WP_000285784.1	4'-phosphopantetheinyl transferase AcpT	-

Sequence

Protein

• Length: 254 a.a.
• Molecular weight: 26833.42 Da
• Isoelectric Point: 6.5992

>NTDB_id=326568 FNE80_RS01240 WP_001136236.1 272865..273629(-) (amiE) [Escherichia coli strain STEC005]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide

• Length: 765 bp

>NTDB_id=326568 FNE80_RS01240 WP_001136236.1 272865..273629(-) (amiE) [Escherichia coli strain STEC005]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (20-168)

3D structure

• AlphaFold DB: A7ZT17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus thermophilus LMG 18311	41.6	98.425	0.409
amiE	Streptococcus thermophilus LMD-9	41.6	98.425	0.409
amiE	Streptococcus salivarius strain HSISS4	40.4	98.425	0.398