Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EGX95_RS20820 Genome accession   NZ_CP033795
Coordinates   3500127..3502466 (-) Length   779 a.a.
NCBI ID   WP_000941259.1    Uniprot ID   A0A5M9GYV1
Organism   Bacillus sp. FDAARGOS_527     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3495127..3507466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGX95_RS30505 - 3495460..3495636 (-) 177 WP_001242605.1 hypothetical protein -
  EGX95_RS30510 - 3495751..3495891 (-) 141 WP_000271105.1 hypothetical protein -
  EGX95_RS20800 (EGX95_20785) - 3495891..3496586 (-) 696 WP_000447391.1 alpha/beta fold hydrolase -
  EGX95_RS20805 (EGX95_20790) - 3496715..3497155 (-) 441 WP_001266956.1 D-tyrosyl-tRNA(Tyr) deacylase -
  EGX95_RS20810 (EGX95_20795) relA 3497173..3499356 (-) 2184 WP_001262809.1 GTP diphosphokinase -
  EGX95_RS20815 (EGX95_20800) - 3499567..3500079 (-) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  EGX95_RS20820 (EGX95_20805) recJ 3500127..3502466 (-) 2340 WP_000941259.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EGX95_RS20825 (EGX95_20810) - 3502568..3503461 (-) 894 WP_001993152.1 cation diffusion facilitator family transporter -
  EGX95_RS20830 (EGX95_20815) secDF 3503768..3506032 (-) 2265 WP_001119088.1 protein translocase subunit SecDF -
  EGX95_RS20835 (EGX95_20820) - 3506303..3506587 (-) 285 WP_000886980.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88139.68 Da        Isoelectric Point: 4.9315

>NTDB_id=325763 EGX95_RS20820 WP_000941259.1 3500127..3502466(-) (recJ) [Bacillus sp. FDAARGOS_527]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADTILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMSLRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANLAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=325763 EGX95_RS20820 WP_000941259.1 3500127..3502466(-) (recJ) [Bacillus sp. FDAARGOS_527]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTATTATATCTTGCCTTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTCCGTTGGGCGC
ATAGCGCAGGATTCTCATTAATTATTACTGTCGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTAATTATTACAGATCACCATGAGCCACCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCCGAAGAAGCGAAAGAGCTGGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAGGTGGAAAATAATTTCCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGGTGGAATCCAGGTGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGCC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAACAGGCAGATACAATTTTAACGGAAGAAGATTTTATTCCAATTACAG
CTGTCGATGCTTTTTGCAAAGTGGAGGACGTAACACTTGCAGCAATTGAAGATATGCAAAAGTTAGCGCCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCAGTGAAAGATGCGGAGTTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
AAAGATGTCTCTTCGTGATGGACAAGCAACATTGGATACAATTGGATTCGGTTTTGGTGCGTACGCGAAAGAAATTTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATCACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9GYV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.972

99.358

0.526

Multiple sequence alignment