Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   D9Y32_RS00395 Genome accession   NZ_CP033218
Coordinates   79524..83024 (-) Length   1166 a.a.
NCBI ID   WP_009328899.1    Uniprot ID   -
Organism   Bacillus licheniformis strain TCCC 11148     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 74524..88024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9Y32_RS00385 (D9Y32_00385) sbcD 74599..75780 (-) 1182 WP_073426309.1 exonuclease subunit SbcD -
  D9Y32_RS00390 (D9Y32_00390) addA 75845..79537 (-) 3693 WP_011197743.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  D9Y32_RS00395 (D9Y32_00395) addB 79524..83024 (-) 3501 WP_009328899.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  D9Y32_RS00400 (D9Y32_00400) modB 83134..83790 (-) 657 WP_009328900.1 molybdate ABC transporter permease subunit -
  D9Y32_RS00405 (D9Y32_00405) modA 83799..84626 (-) 828 WP_392387299.1 molybdate ABC transporter substrate-binding protein -
  D9Y32_RS00410 (D9Y32_00410) - 84796..85234 (+) 439 Protein_83 ferritin-like domain-containing protein -
  D9Y32_RS00415 (D9Y32_00415) - 85357..85998 (+) 642 WP_044051832.1 response regulator transcription factor -
  D9Y32_RS00420 (D9Y32_00420) - 85995..87362 (+) 1368 WP_011197741.1 cell wall metabolism sensor histidine kinase WalK -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134408.49 Da        Isoelectric Point: 5.5700

>NTDB_id=322691 D9Y32_RS00395 WP_009328899.1 79524..83024(-) (addB) [Bacillus licheniformis strain TCCC 11148]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLPPQSIRDMMEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYAEEIRNAAVYVDGFHQFTPQEMSVLEQLMVHAEEITFSLTADKP
FTANSPNDLHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHRHHPELQHLERYFDERPAKPYPGQTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEEGGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDEDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDSEEKMLIHEPEQLDDEAQLEFLVNKG
IALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNIISAGLKKDGSLRSDSDVVAEEDFHVLRRHIRRTFQQAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDSVILERLKKEDEADGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=322691 D9Y32_RS00395 WP_009328899.1 79524..83024(-) (addB) [Bacillus licheniformis strain TCCC 11148]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCAGATTTGGGCGGCACCATCCGCGCCCAAGTGTACAGTTTTTCACGGCTTGCCTGGCGAGTGCTCCAACACACCGGA
GGCATGAACCGGCCGTTCTTGACGGGAACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCACT
GTCTGCCGCCTCAAAGCATCCGGGATATGATGGAAGGGACAGGCAAGTCGGAATACGAAGATGAACGGATTCTTTCCGAT
AAATTGCACGACTTATATATTTTGTACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAAGATTATTT
GACCCTTTTAGCCGAACAGATTCCGTATGCGGAAGAGATTAGAAACGCTGCCGTTTACGTTGACGGATTTCACCAATTCA
CGCCTCAGGAGATGAGCGTACTAGAACAGCTGATGGTACATGCCGAAGAGATCACGTTTTCGCTGACGGCGGATAAGCCG
TTTACGGCAAACAGCCCGAATGATCTCCATCTGTTCAGGATGACCGGGAAGGCGTACTATGACCTTTACCAAAAGGCGAA
GGAACTCGGCCTTGATCCGTCTGAAGTGCGGCTGGAAGAAACGAAACGGCACCGGCATCATCCGGAGCTTCAGCATCTTG
AACGCTATTTTGATGAACGTCCTGCAAAACCGTATCCCGGACAGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATCCGCCAGGGCCGGTTCCGGCTTAGGGATATTGCTGT
CATCGCCCGCAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGCGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTTATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAATTGGCGGTAT
GAAGCCGTGTTCCGCTGTGTCAAAACGGAGCTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCTTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGAT
ACGCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCGCCGATTCACAGGCTGCAGAAGCGATTGAAAAAAGCTGAAACAGTAAGGGAAATGGCCGAAGCCGTCTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTAGCCGAAGAAGGCGGAAGGATTGCCGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATT
TCGTTTGCGTTATTTCAGCAAATGATCGAAACCGGATTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCACTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGTACATTTCTCATCGGTGTAAACGACGGCATAC
TTCCGGCGAGGCCTGCGGATGACGGCGTGCTTTCAGACGAGGACAGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCG
GCAACAGGGCGAGAGCAGCTATTGGACGAGAATTTTCTGATCTATATGACGCTTTCAAGCCCGTCTGAAAAGCTTTATGT
CTCCTATCCGATCGCTGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTGGTGAAACGGCTGAATGAACTGTTCCCGG
ACAGCGAAGAAAAGATGCTGATTCATGAACCCGAGCAGCTGGACGATGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
ATCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATCTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAACATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACCTAAACCGGCATGTGAGCCGCGATTTATACGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAGGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGCTTAAAGCTGAAGGAACGCCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCTGCGCTGAAGCTGATTTCAGACCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAGTGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTTGCGCCTAAACTGCAAAAGGAGATTCTGCTCAGTTCGAAC
CGTCATCATTATGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGTGAGCATGCAAAAGCAAG
CGGCTTTGCCCCCGTCGGCATAGAGCTCGGATTCGGAGGGAAAGGTCCGCTTCCTCCAATGCGTTTCACCTTGAAGAACG
GTTGCACGATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGTTGCTGCTTAGGATTGTT
GATTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGGCTTGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACGCATTCAACCGATTGGCTCGGCATGAAAGCATCCCCGGCAGGCGTGCTCTATTTTCATGTGCACGATC
CAATGATCCAGGCTTCAGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTA
CTCCTGGGAGACCAGGAAGCGGTCAAGCTGATGGACACCACCCTGGAACAAGGCAGGTCGAATATTATCAGCGCCGGTTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTGTTGCAGAAGAGGATTTTCATGTATTGAGGCGTCATATCCGCC
GGACATTTCAGCAGGCCGGCGAGGAGATCACAGACGGCAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCATACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGAATATTAAAACCTGA
AAAAGACAGCGTGATATTGGAGCGGCTGAAAAAGGAGGATGAGGCAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

74.077

99.914

0.74

Multiple sequence alignment