Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LLKF_RS03260 Genome accession   NC_013656
Coordinates   626072..628300 (+) Length   742 a.a.
NCBI ID   WP_012897342.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis KF147     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 621072..633300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLKF_RS03225 (LLKF_0621) - 621561..622229 (+) 669 WP_003129542.1 hypothetical protein -
  LLKF_RS03230 (LLKF_0622) - 622399..623295 (+) 897 WP_003129543.1 SPFH domain-containing protein -
  LLKF_RS03240 (LLKF_0623) - 623670..623930 (+) 261 WP_003129544.1 SemiSWEET family transporter -
  LLKF_RS03250 (LLKF_0624) rnz 624288..625211 (+) 924 WP_012897340.1 ribonuclease Z -
  LLKF_RS03255 (LLKF_0625) - 625204..625905 (+) 702 WP_012897341.1 SDR family NAD(P)-dependent oxidoreductase -
  LLKF_RS03260 (LLKF_0626) recJ 626072..628300 (+) 2229 WP_012897342.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LLKF_RS03265 (LLKF_0627) - 628423..628935 (+) 513 WP_012897343.1 adenine phosphoribosyltransferase -
  LLKF_RS03270 (LLKF_0628) rpoE 629151..629714 (+) 564 WP_012897344.1 DNA-directed RNA polymerase subunit delta -
  LLKF_RS03275 (LLKF_0629) mltG 629851..631485 (+) 1635 WP_012897345.1 endolytic transglycosylase MltG -
  LLKF_RS03280 (LLKF_0630) greA 631545..632018 (+) 474 WP_025016857.1 transcription elongation factor GreA -
  LLKF_RS03285 (LLKF_0631) - 632195..632650 (+) 456 WP_010905471.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83539.67 Da        Isoelectric Point: 4.7316

>NTDB_id=32065 LLKF_RS03260 WP_012897342.1 626072..628300(+) (recJ) [Lactococcus lactis subsp. lactis KF147]
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGVHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTAASVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEEGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEVVKKAMIDYIVDNHLDMSKKSPLEIADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWDGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEETPGDLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=32065 LLKF_RS03260 WP_012897342.1 626072..628300(+) (recJ) [Lactococcus lactis subsp. lactis KF147]
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGCCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAGTTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGACCCTTTTCTACTTCATGATATGGAAAAAGCAACAGGGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAATATTTTAATTTATGGTGACTATGATGCTGACGGAATGACTGCAGCATCAGTCATGAAGTCCGCTCTGGATGA
ATTAGGAGCTGAGGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCAAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGGGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACCGACCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
TGAACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGTGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTAGTTGCAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGTCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATGAAGTATGCCGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCCCGTTTAAACGCTTTGGGACGTCTCGATGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGTTAGAAGCTATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGAGGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGGATGACTTTAAGTCTGGAAAACGTAGAAGTAGTTAA
AAAGGCGATGATTGATTATATCGTGGATAATCATTTAGATATGTCTAAAAAAAGTCCCTTAGAAATTGCTGACCGTTGCC
ACTTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCTCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGGTGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAGGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAACAGGCACAAACAAAATTAG
TCGCAACACTTTCAAGTAATAGTTGGGATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCTAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGACACCTGGAGATTTAGCAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGTCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
AGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGCCGATAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Domains

Predicted by InterProScan.

DHHA1 domain (DHHA1)

(317-434)

RecJ OB domain (RecJ_OB)

(454-554)

DHH family (DHH)

(83-228)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(592-737)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

56.644

100

0.569
  recJ Bacillus subtilis subsp. subtilis str. 168

36.457

100

0.383

Multiple sequence alignment