Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   E6P00_RS28070 Genome accession   NZ_CP040127
Coordinates   5374772..5375197 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PA298     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5369772..5380197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6P00_RS28055 (E6P00_28055) pilX 5370336..5370923 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  E6P00_RS28060 (E6P00_28060) pilY1 5370935..5374426 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  E6P00_RS28065 (E6P00_28065) pilY2 5374428..5374775 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  E6P00_RS28070 (E6P00_28070) comF 5374772..5375197 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  E6P00_RS28075 (E6P00_28075) ispH 5375244..5376188 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  E6P00_RS28080 (E6P00_28080) fkpB 5376274..5376714 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  E6P00_RS28085 (E6P00_28085) lspA 5376707..5377216 (-) 510 WP_003110420.1 signal peptidase II -
  E6P00_RS28090 (E6P00_28090) ileS 5377209..5380040 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=320154 E6P00_RS28070 WP_003094721.1 5374772..5375197(+) (comF) [Pseudomonas aeruginosa strain PA298]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=320154 E6P00_RS28070 WP_003094721.1 5374772..5375197(+) (comF) [Pseudomonas aeruginosa strain PA298]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment